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Effects of ultraviolet light on the in vitro assembly of microtubules

Exposure of microtubular protein to ultraviolet light inhibits its assembly into morphologically normal microtubules. This effect appeared to result primarily from damage to the tubulin dimers. The damage consisted of a conformational change, a loss of two free sulfhydryl groups, a production of hig...

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Published in:	Biochemistry (Easton) 1984-03, Vol.23 (6), p.1073-1080
Main Authors:	Zaremba, Terrye G, LeBon, Thomas R, Millar, David B, Smejkal, Ruthann M, Hawley, Robert J
Format:	Article
Language:	English
Subjects:	Animals Biological and medical sciences Brain - ultrastructure Cattle Circular Dichroism Fundamental and applied biological sciences. Psychology Interphase Microscopy, Electron Microtubules - radiation effects Molecular biophysics Nephelometry and Turbidimetry Polymers - metabolism Structure in molecular biology Sulfuric Acid Esters - metabolism Supramolecular structure Tubulin - radiation effects Ultracentrifugation Ultraviolet Rays
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container_end_page	1080
container_issue	6
container_start_page	1073
container_title	Biochemistry (Easton)
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creator	Zaremba, Terrye G LeBon, Thomas R Millar, David B Smejkal, Ruthann M Hawley, Robert J
description	Exposure of microtubular protein to ultraviolet light inhibits its assembly into morphologically normal microtubules. This effect appeared to result primarily from damage to the tubulin dimers. The damage consisted of a conformational change, a loss of two free sulfhydryl groups, a production of higher molecular weight cross-linked species, and the formation of aggregated amorphous material upon polymerization.
doi_str_mv	10.1021/bi00301a006
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source	ACS CRKN Legacy Archives
subjects	Animals Biological and medical sciences Brain - ultrastructure Cattle Circular Dichroism Fundamental and applied biological sciences. Psychology Interphase Microscopy, Electron Microtubules - radiation effects Molecular biophysics Nephelometry and Turbidimetry Polymers - metabolism Structure in molecular biology Sulfuric Acid Esters - metabolism Supramolecular structure Tubulin - radiation effects Ultracentrifugation Ultraviolet Rays
title	Effects of ultraviolet light on the in vitro assembly of microtubules
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