Reactions of Mycobacterium tuberculosis Truncated Hemoglobin O with Ligands Reveal a Novel Ligand-Inclusive Hydrogen Bond Network

Truncated hemoglobin O (trHbO) is one of two trHbs in Mycobacterium tuberculosis. Remarkably, trHbO possesses two novel distal residues, in addition to the B10 tyrosine, that may be important in ligand binding. These are the CD1 tyrosine and G8 tryptophan. Here we investigate the reactions of trHbO...

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Published in:Biochemistry (Easton) 2003-05, Vol.42 (19), p.5764-5774
Main Authors: Ouellet, Hugues, Juszczak, Laura, Dantsker, David, Samuni, Uri, Ouellet, Yannick H, Savard, Pierre-Yves, Wittenberg, Jonathan B, Wittenberg, Beatrice A, Friedman, Joel M, Guertin, Michel
Format: Article
Language:English
Subjects:
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Binding Sites
Carbon Monoxide - metabolism
Heme - chemistry
Hemoglobins - chemistry
Hemoglobins - genetics
Hemoglobins - metabolism
Hydrogen Bonding
Kinetics
Ligands
Models, Molecular
Mutagenesis, Site-Directed
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - metabolism
Nitric Oxide - metabolism
Oxygen - metabolism
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Truncated Hemoglobins
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cited_by cdi_FETCH-LOGICAL-a349t-e65a1e2dd4ba40f8b6f31c6bd18fb4d08c58cc9f5686e47c07255e98306118773
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container_issue 19
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creator Ouellet, Hugues
Juszczak, Laura
Dantsker, David
Samuni, Uri
Ouellet, Yannick H
Savard, Pierre-Yves
Wittenberg, Jonathan B
Wittenberg, Beatrice A
Friedman, Joel M
Guertin, Michel
description Truncated hemoglobin O (trHbO) is one of two trHbs in Mycobacterium tuberculosis. Remarkably, trHbO possesses two novel distal residues, in addition to the B10 tyrosine, that may be important in ligand binding. These are the CD1 tyrosine and G8 tryptophan. Here we investigate the reactions of trHbO and mutants using stopped-flow spectrometry, flash photolysis, and UV-enhanced resonance Raman spectroscopy. A biphasic kinetic behavior is observed for combination and dissociation of O2 and CO that is controlled by the B10 and CD1 residues. The rate constants for combination (
doi_str_mv 10.1021/bi0270337
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Remarkably, trHbO possesses two novel distal residues, in addition to the B10 tyrosine, that may be important in ligand binding. These are the CD1 tyrosine and G8 tryptophan. Here we investigate the reactions of trHbO and mutants using stopped-flow spectrometry, flash photolysis, and UV-enhanced resonance Raman spectroscopy. A biphasic kinetic behavior is observed for combination and dissociation of O2 and CO that is controlled by the B10 and CD1 residues. The rate constants for combination (&lt;1.0 μM-1 s-1) and dissociation (&lt;0.006 s-1) of O2 are among the slowest known, precluding transport or diffusion of O2 as a major function. Mutation of CD1 tyrosine to phenylalanine shows that this group controls ligand binding, as evidenced by 25- and 77-fold increases in the combination rate constants for O2 and CO, respectively. In support of a functional role for G8 tryptophan, UV resonance Raman indicates that the χ(2,1) dihedral angle for the indole ring increases progressively from approximately 93° to at least 100° in going sequentially from the deoxy to CO to O2 derivative, demonstrating a significant conformational change in the G8 tryptophan with ligation. Remarkably, protein modeling predicts a network of hydrogen bonds between B10 tyrosine, CD1 tyrosine, and G8 tryptophan, with the latter residues being within hydrogen bonding distance of the heme-bound ligand. Such a rigid hydrogen bonding network may thus represent a considerable barrier to ligand entrance and escape. In accord with this model, we found that changing CD1 or B10 tyrosine for phenylalanine causes only small changes in the rate of O2 dissociation, suggesting that more than one hydrogen bond must be broken at a time to promote ligand escape. 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Remarkably, trHbO possesses two novel distal residues, in addition to the B10 tyrosine, that may be important in ligand binding. These are the CD1 tyrosine and G8 tryptophan. Here we investigate the reactions of trHbO and mutants using stopped-flow spectrometry, flash photolysis, and UV-enhanced resonance Raman spectroscopy. A biphasic kinetic behavior is observed for combination and dissociation of O2 and CO that is controlled by the B10 and CD1 residues. The rate constants for combination (&lt;1.0 μM-1 s-1) and dissociation (&lt;0.006 s-1) of O2 are among the slowest known, precluding transport or diffusion of O2 as a major function. Mutation of CD1 tyrosine to phenylalanine shows that this group controls ligand binding, as evidenced by 25- and 77-fold increases in the combination rate constants for O2 and CO, respectively. In support of a functional role for G8 tryptophan, UV resonance Raman indicates that the χ(2,1) dihedral angle for the indole ring increases progressively from approximately 93° to at least 100° in going sequentially from the deoxy to CO to O2 derivative, demonstrating a significant conformational change in the G8 tryptophan with ligation. Remarkably, protein modeling predicts a network of hydrogen bonds between B10 tyrosine, CD1 tyrosine, and G8 tryptophan, with the latter residues being within hydrogen bonding distance of the heme-bound ligand. Such a rigid hydrogen bonding network may thus represent a considerable barrier to ligand entrance and escape. In accord with this model, we found that changing CD1 or B10 tyrosine for phenylalanine causes only small changes in the rate of O2 dissociation, suggesting that more than one hydrogen bond must be broken at a time to promote ligand escape. Furthermore, trHbO−CO cannot be photodissociated under conditions where the CO derivative of myoglobin is extensively photodissociated, indicating that CO is constrained near the heme by the hydrogen bonding network.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>12741834</pmid><doi>10.1021/bi0270337</doi><tpages>11</tpages></addata></record>
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Binding Sites
Carbon Monoxide - metabolism
Heme - chemistry
Hemoglobins - chemistry
Hemoglobins - genetics
Hemoglobins - metabolism
Hydrogen Bonding
Kinetics
Ligands
Models, Molecular
Mutagenesis, Site-Directed
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - metabolism
Nitric Oxide - metabolism
Oxygen - metabolism
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Truncated Hemoglobins
title Reactions of Mycobacterium tuberculosis Truncated Hemoglobin O with Ligands Reveal a Novel Ligand-Inclusive Hydrogen Bond Network
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