Characterization of Interactions of 4-Nitrophenylpropyl-N-alkylamine with Sigma Receptors

• Published in: Biochemistry (Easton) 2011-09, Vol.50 (35), p.7568-7578
• Main Authors: Chu, Uyen B, Hajipour, Abdol R, Ramachandran, Subramaniam, Ruoho, Arnold E
• Format: Article
• Language: English
• Subjects: Amines - chemistry; Amines - metabolism; Animals; Cell Line, Tumor; Guinea Pigs; HEK293 Cells; Humans; Ligands; Liver - chemistry; Liver - metabolism; Nitrophenols - chemistry; Nitrophenols - metabolism; Protein Binding; Protein Interaction Mapping; Rats; Receptors, sigma - chemistry; Receptors, sigma - metabolism; Sigma-1 Receptor
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi2004872

Sigma receptors are small membrane proteins implicated in a number of pathophysiological conditions, including drug addiction, psychosis, and cancer; thus, small molecule inhibitors of sigma receptors have been proposed as potential pharmacotherapeutics for these diseases. We previously discovered that endogenous monochain N-alkyl sphingolipids, including d-erythro-sphingosine, sphinganine, and N,N-dimethylsphingosine, bind to the sigma-1 receptor at physiologically relevant concentrations [Ramachandran, S., et al. (2009) Eur. J. Pharmacol. 609, 19–26]. Here, we investigated several N-alkylamines of varying chain lengths as sigma receptor ligands. Although the K I values for N-alkylamines were found to be in the micromolar range, when N-3-phenylpropyl and N-3-(4-nitrophenyl)­propyl derivatives of butylamine (1a and 1b, respectively), heptylamine (2a and 2b, respectively), dodecylamine (3a and 3b, respectively), and octadecylamine (4a and 4b, respectively) were evaluated as sigma receptor ligands, we found that these compounds exhibited nanomolar affinities with both sigma-1 and sigma-2 receptors. A screen of high-affinity ligands 2a, 2b, 3a, and 3b against a variety of other receptors and/or transporters confirmed these four compounds to be highly selective mixed sigma-1 and sigma-2 ligands. Additionally, in HEK-293 cells reconstituted with Kv1.4 potassium channel and the sigma-1 receptor, these derivatives were able to inhibit the outward current from the channel, consistent with sigma receptor modulation. Finally, cytotoxicity assays showed that 2a, 2b, 3a, and 3b were highly potent against a number of cancer cell lines, demonstrating their potential utility as mixed sigma-1 and sigma-2 receptor anticancer agents.