Phase-Shifting Interferometry for the Study of the Step Dynamics during Crystallization of Proteins

We have developed a novel phase-shifting interferometry technique for high-resolution in-situ investigations of the unsteady dynamics of growth steps during the crystallization of proteins. The phase-shifting algorithm employs five-image sequences captured with a phase shift of π/2; digital processi...

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Bibliographic Details
Published in:Crystal growth & design 2002-09, Vol.2 (5), p.381-385
Main Authors: Gliko, Olga, Booth, Nicholas A, Rosenbach, Eva, Vekilov, Peter G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cross-disciplinary physics: materials science
rheology
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
In solution. Condensed state. Thin layers
Materials science
Methods of crystal growth
physics of crystal growth
Molecular biophysics
Physico-chemical properties of biomolecules
Physics
Theory and models of crystal growth
physics of crystal growth, crystal morphology and orientation
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Summary:We have developed a novel phase-shifting interferometry technique for high-resolution in-situ investigations of the unsteady dynamics of growth steps during the crystallization of proteins. The phase-shifting algorithm employs five-image sequences captured with a phase shift of π/2; digital processing of the sequence allows reconstruction of the surface morphology with a depth resolution
ISSN:1528-7483
1528-7505
DOI:10.1021/cg020013j