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Phase-Shifting Interferometry for the Study of the Step Dynamics during Crystallization of Proteins
We have developed a novel phase-shifting interferometry technique for high-resolution in-situ investigations of the unsteady dynamics of growth steps during the crystallization of proteins. The phase-shifting algorithm employs five-image sequences captured with a phase shift of π/2; digital processi...
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Published in: | Crystal growth & design 2002-09, Vol.2 (5), p.381-385 |
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container_end_page | 385 |
container_issue | 5 |
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container_title | Crystal growth & design |
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creator | Gliko, Olga Booth, Nicholas A Rosenbach, Eva Vekilov, Peter G |
description | We have developed a novel phase-shifting interferometry technique for high-resolution in-situ investigations of the unsteady dynamics of growth steps during the crystallization of proteins. The phase-shifting algorithm employs five-image sequences captured with a phase shift of π/2; digital processing of the sequence allows reconstruction of the surface morphology with a depth resolution |
doi_str_mv | 10.1021/cg020013j |
format | article |
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The phase-shifting algorithm employs five-image sequences captured with a phase shift of π/2; digital processing of the sequence allows reconstruction of the surface morphology with a depth resolution <5 nm and a lateral resolution of 0.5 μm across a field of view as wide as 1 mm. Such sequences can be recorded with a frequency of ∼1 s-1 and allow monitoring of the appearance and evolution of local morphology features, such as step bunches. Time traces of the variations of the growth rate and local slope (proportional to the density of the growth steps) at up to 10 select locations on a studied crystals facet are recorded with time resolution that can be as low as 0.2 s. Application of this technique to the ferritin crystals shows extensive fluctuations of growth rate and local slope as a result of step bunching.</description><identifier>ISSN: 1528-7483</identifier><identifier>EISSN: 1528-7505</identifier><identifier>DOI: 10.1021/cg020013j</identifier><language>eng</language><publisher>Washington,DC: American Chemical Society</publisher><subject>Biological and medical sciences ; Cross-disciplinary physics: materials science; rheology ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; In solution. Condensed state. 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Application of this technique to the ferritin crystals shows extensive fluctuations of growth rate and local slope as a result of step bunching.</description><subject>Biological and medical sciences</subject><subject>Cross-disciplinary physics: materials science; rheology</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In solution. Condensed state. Thin layers</subject><subject>Materials science</subject><subject>Methods of crystal growth; physics of crystal growth</subject><subject>Molecular biophysics</subject><subject>Physico-chemical properties of biomolecules</subject><subject>Physics</subject><subject>Theory and models of crystal growth; physics of crystal growth, crystal morphology and orientation</subject><issn>1528-7483</issn><issn>1528-7505</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNptkEtLAzEUhYMoWKsL_0E2LlyM3pnczGMp9VUoWKiuhzSPNmWalCRdjL_ella7cXXuge8cuIeQ2xwecijyR7mAAiBnqzMyyHlRZxUHfv57Y80uyVWMKwCoSsYGRE6XIupstrQmWbegY5d0MDr4tU6hp8YHmpaaztJW9dSbo9Eb-tw7sbYyUrUN--Ao9DGJrrPfIlnv9uw0-KSti9fkwogu6pujDsnX68vn6D2bfLyNR0-TTDDOUmY4k4bVBhUWiEzKRhmuNJ9jVSLKulFQVaDmRjUazRzyUivV1FAgA1QNsiG5P_TK4GMM2rSbYNci9G0O7X6d9m-dHXt3YDciStGZIJy08RRABF421YkTMrYrvw1u98E_fT8EF3HF</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>Gliko, Olga</creator><creator>Booth, Nicholas A</creator><creator>Rosenbach, Eva</creator><creator>Vekilov, Peter G</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20020901</creationdate><title>Phase-Shifting Interferometry for the Study of the Step Dynamics during Crystallization of Proteins</title><author>Gliko, Olga ; Booth, Nicholas A ; Rosenbach, Eva ; Vekilov, Peter G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a353t-f53cf38f4d42443cc9df5de5b47644c89d0770dbfd9e4fb016edd98024304d943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Cross-disciplinary physics: materials science; rheology</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In solution. Condensed state. Thin layers</topic><topic>Materials science</topic><topic>Methods of crystal growth; physics of crystal growth</topic><topic>Molecular biophysics</topic><topic>Physico-chemical properties of biomolecules</topic><topic>Physics</topic><topic>Theory and models of crystal growth; physics of crystal growth, crystal morphology and orientation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gliko, Olga</creatorcontrib><creatorcontrib>Booth, Nicholas A</creatorcontrib><creatorcontrib>Rosenbach, Eva</creatorcontrib><creatorcontrib>Vekilov, Peter G</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Crystal growth & design</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gliko, Olga</au><au>Booth, Nicholas A</au><au>Rosenbach, Eva</au><au>Vekilov, Peter G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phase-Shifting Interferometry for the Study of the Step Dynamics during Crystallization of Proteins</atitle><jtitle>Crystal growth & design</jtitle><addtitle>Cryst. 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Time traces of the variations of the growth rate and local slope (proportional to the density of the growth steps) at up to 10 select locations on a studied crystals facet are recorded with time resolution that can be as low as 0.2 s. Application of this technique to the ferritin crystals shows extensive fluctuations of growth rate and local slope as a result of step bunching.</abstract><cop>Washington,DC</cop><pub>American Chemical Society</pub><doi>10.1021/cg020013j</doi><tpages>5</tpages></addata></record> |
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subjects | Biological and medical sciences Cross-disciplinary physics: materials science rheology Exact sciences and technology Fundamental and applied biological sciences. Psychology In solution. Condensed state. Thin layers Materials science Methods of crystal growth physics of crystal growth Molecular biophysics Physico-chemical properties of biomolecules Physics Theory and models of crystal growth physics of crystal growth, crystal morphology and orientation |
title | Phase-Shifting Interferometry for the Study of the Step Dynamics during Crystallization of Proteins |
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