Versatile and Efficient Immobilization of 2‑Deoxyribose-5-phosphate Aldolase (DERA) on Multiwalled Carbon Nanotubes

A series of five new catalysts I–V based upon the immobilization of 2-deoxyribose-5-phosphate aldolase (DERA) on multiwalled carbon nanotubes (MWCNTs) were prepared, and their use for the formation of the C–C bond via cross-aldol condensation, under environmental friendly conditions, was studied. Am...

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Published in:ACS catalysis 2014-09, Vol.4 (9), p.3059-3068
Main Authors: Subrizi, Fabiana, Crucianelli, Marcello, Grossi, Valentina, Passacantando, Maurizio, Botta, Giorgia, Antiochia, Riccarda, Saladino, Raffaele
Format: Article
Language:English
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Summary:A series of five new catalysts I–V based upon the immobilization of 2-deoxyribose-5-phosphate aldolase (DERA) on multiwalled carbon nanotubes (MWCNTs) were prepared, and their use for the formation of the C–C bond via cross-aldol condensation, under environmental friendly conditions, was studied. Among the various employed procedures, the direct immobilization of the enzyme through ionic exchange interactions on oxidized multiwalled carbon nanotubes (ox-MWCNTs) afforded the most efficient catalyst I. This catalyst was shown to be stable with a high tolerance to acetaldehyde, and it maintained its activity for several days, being reused for five runs. The versatility of catalyst I in cross-aldol condensation was evaluated in the reaction of acetaldehyde alone (self-condensation) or in the presence of chloro-acetaldehyde. The corresponding cyclic lactols were obtained in higher yield than native DERA.
ISSN:2155-5435
2155-5435
DOI:10.1021/cs500511c