A Bioaccumulative Cyclometalated Platinum(II) Complex with Two-Photon-Induced Emission for Live Cell Imaging

The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing Cphenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the...

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Bibliographic Details
Published in:Inorganic chemistry 2009-02, Vol.48 (3), p.872-878
Main Authors: Koo, Chi-Kin, Wong, Ka-Leung, Man, Cornelia Wing-Yin, Lam, Yun-Wah, So, Leo King-Yan, Tam, Hoi-Lam, Tsao, Sai-Wah, Cheah, Kok-Wai, Lau, Kai-Chung, Yang, Yang-Yi, Chen, Jin-Can, Lam, Michael Hon-Wah
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cell Survival - drug effects
Cyclization
Humans
Mice
Molecular Structure
Photochemistry
Photons
Platinum Compounds - chemistry
Platinum Compounds - toxicity
Spectrophotometry
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Summary:The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing Cphenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.
ISSN:0020-1669
1520-510X
DOI:10.1021/ic801261h