Monitoring the Disassembly of Virus-like Particles by 19 F-NMR

Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2017-04, Vol.139 (15), p.5277-5280
Main Authors: Leung, Rogen L C, Robinson, Matthew D M, Ajabali, Alaa A A, Karunanithy, Gogulan, Lyons, Brian, Raj, Ritu, Raoufmoghaddam, Saeed, Mohammed, Shabaz, Claridge, Timothy D W, Baldwin, Andrew J, Davis, Benjamin G
Format: Article
Language:English
Subjects:
Bacteriophage lambda - chemistry
Fluorine - chemistry
Nuclear Magnetic Resonance, Biomolecular
Vaccines, Virus-Like Particle - analysis
Viral Proteins - chemistry
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Summary:Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly of F-labeled VLPs derived from bacteriophage Qβ by F NMR. Analysis of resonances, under a range of conditions, allowed determination not only of the particle as fully assembled but also as disassembled, as well as detection of a degraded state upon digestion by cells. This in turn allowed mutational redesign of disassembly and testing in both bacterial and mammalian systems as a strategy for the creation of putative, targeted-VLP delivery systems.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.6b11040