Oxidative damage of kidney, heart, lung, and spleen measured by oxidized heme proteins in tissue slices

Oxidative damage to rat kidney, heart, lung, and spleen was measured by the formation of oxidized heme proteins (OHP) in tissue slices. Kidney, heart, lung, and spleen slices were incubated in oxygenated KRP-glucose buffer at 37 degrees C with and without the presence of prooxidants. The absorbance...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1993-10, Vol.41 (10), p.1640-1643
Main Authors: Chen, Hao, Tappel, Al L
Format: Article
Language:English
Subjects:
BAZO
Biological and medical sciences
COEUR
CORAZON
Diverse techniques
ESPECTROMETRIA
Fundamental and applied biological sciences. Psychology
HEMOGLOBINA
HEMOGLOBINE
METALLOPROTEINE
METALPROTEINAS
Molecular and cellular biology
OXIDACION
OXYDATION
POUMON
PROTEINAS
PROTEINE
PULMONES
RAT
RATA
RATE
REIN
RINONES
SPECTROMETRIE
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Summary:Oxidative damage to rat kidney, heart, lung, and spleen was measured by the formation of oxidized heme proteins (OHP) in tissue slices. Kidney, heart, lung, and spleen slices were incubated in oxygenated KRP-glucose buffer at 37 degrees C with and without the presence of prooxidants. The absorbance spectra (500-640 nm) of heme proteins in the fresh and oxidized tissue slices were analyzed, and the concentrations of OHP, such as methemoglobin, ferrylhemoglobin, hemichrome, oxidized mitochondrial, and microsomal cytochromes were determined with a heme protein spectra analysis program. Bromotrichloromethane (CBrCl3), tert-butyl hydroperoxide (TBHP), ferrous sulfate (FeSO4), and cupric chloride (CuCl2) accelerated the oxidative reactions, and the amount of OHP was dependent on the incubation time as well as the type and concentration of prooxidants
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00034a023