Size Exclusion Chromatography of Soybean Proteins and Isoflavones

High-performance size exclusion chromatography was used to separate soybean extracts. The separation of the major storage proteins, including glycinin and beta-conglycinin, was determined by analysis of the subunits on sodium dodecyl sulfate gel electrophoresis. The extracts of defatted flour and wh...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1994-12, Vol.42 (12), p.2713-2720
Main Authors: Cole, Kenneth D, Cousin, Sydney L
Format: Article
Language:English
Subjects:
Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts
Biological and medical sciences
CHROMATOGRAPHIE
CROMATOGRAFIA
FLAVONOIDE
FLAVONOIDES
Food industries
Fundamental and applied biological sciences. Psychology
GLUCOSIDE
GLUCOSIDOS
PROTEINAS VEGETALES
PROTEINE VEGETALE
SEPARACION
SEPARATION
SOJA
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Summary:High-performance size exclusion chromatography was used to separate soybean extracts. The separation of the major storage proteins, including glycinin and beta-conglycinin, was determined by analysis of the subunits on sodium dodecyl sulfate gel electrophoresis. The extracts of defatted flour and whole beans showed a high degree of polymerization of the storage proteins. Addition of a disulfide reducing agent (dithiothreitol) resulted in a decrease of the polymerized forms. The separation of the major glucoside isoflavones (genistin, daidzin, and glycitein 7-beta-glucoside) was determined. The isoflavone glucosides eluted well past the elusion time of a small totally included molecule, indicating that they are separating by mechanisms other than size exclusion. Size exclusion chromatography was used to monitor the separation of the soybean extract in an aqueous two-phase extraction system. The major storage proteins partitioned to the salt phase, and the isoflavones and other hydrophobic compounds partitioned to the poly(ethylene glycol) phase
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00048a013