Characterization of pure proanthocyanidins isolated from the hulls of faba beans

Extraction of the hulls of faba beans with 70% aqueous acetone containing 2.8 mmol/L ascorbic acid proved to be a very efficient solvent system for the extraction of phenols. Treatment of the concentrated extract with ethyl acetate and liquefied phenol and batch adsorption on Sephadex LH-20 freed th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 1990-01, Vol.38 (1), p.95-98
Main Authors: Hussein, Laila, Fattah, Mohy Abdel, Salem, Ezzildin
Format: Article
Language:English
Subjects:
Biological and medical sciences
CHROMATOGRAPHIE
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CROMATOGRAFIA
FEVE
FLAVONOIDE
FLAVONOIDES
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
HABA
PURIFICACION
PURIFICATION
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Extraction of the hulls of faba beans with 70% aqueous acetone containing 2.8 mmol/L ascorbic acid proved to be a very efficient solvent system for the extraction of phenols. Treatment of the concentrated extract with ethyl acetate and liquefied phenol and batch adsorption on Sephadex LH-20 freed the isolated crude proanthocyanidins (PA) from small molecular weight UV-absorbing and protein contaminants. Gel filtration of the purified extract on Sephadex LH-20 yielded pure PA and showed homogeneous band during the elution at 275.2 nm (maximum). The overall yield was 12%; the preparation contained six flavan-3-ol units/molecules
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00091a018