Enzyme-Linked Immunosorbent Assay for the Pyrethroid Deltamethrin

A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of deltamethrin was developed. Two haptens, cyano[3-(4-aminophenoxy)phenyl]methyl 1R-cis-3-(2,2−dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate and 3-[(±)-cyano[1R-cis-3-(2,2−dibromoethenyl)-2,2-dimethylcyclopropan ecarbon...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2002-09, Vol.50 (20), p.5526-5532
Main Authors: Lee, Hu-Jang, Shan, Guomin, Watanabe, Takaho, Stoutamire, Donald W, Gee, Shirley J, Hammock, Bruce D
Format: Article
Language:English
Subjects:
Analysis methods
Animals
Antibody Specificity
Applied sciences
Biological and medical sciences
Enzyme-Linked Immunosorbent Assay
Exact sciences and technology
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
Haptens - chemistry
Hydrogen-Ion Concentration
Immune Sera - immunology
Insecticides - analysis
Magnetic Resonance Spectroscopy
Natural water pollution
Nitriles
Pollution
Pyrethrins - analysis
Pyrethrins - immunology
Rabbits
Sensitivity and Specificity
Water treatment and pollution
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Summary:A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of deltamethrin was developed. Two haptens, cyano[3-(4-aminophenoxy)phenyl]methyl 1R-cis-3-(2,2−dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate and 3-[(±)-cyano[1R-cis-3-(2,2−dibromoethenyl)-2,2-dimethylcyclopropan ecarbonyloxy]methyl]phenoxyacetic acid, were synthesized and conjugated with thyroglobulin as immunogens. Four antisera were generated and screened against six different coating antigens. The assay that was the most sensitive for deltamethrin was optimized and characterized. The I 50 for deltamethrin was 17.5 ± 3.6 μg/L, and the lower detection limit was 1.1 ± 0.5 μg/L. This ELISA assay had relatively low cross-reactivities with other major pyrethroids, such as permethrin, phenothrin, bioresmethrin, cyfluthrin, and cypermethrin. Methanol was found to be the best organic cosolvent for this ELISA, with optimal sensitivity observed at a concentration of 40% (v/v). The assay parameters were unchanged at pH values between 5.0 and 8.0, whereas higher ionic strengths strongly suppressed the absorbances. To increase the sensitivity of the overall method, a C18 sorbent-based solid-phase extraction was used for river water samples. River water samples fortified with deltamethrin were analyzed according to this method. Good recoveries and correlation with spike levels were observed. Keywords: Deltamethrin; ELISA; environmental monitoring; pyrethroid
ISSN:0021-8561
1520-5118
DOI:10.1021/jf0207629