Direct Determination of Paclobutrazol Residues in Pear Samples by Liquid Chromatography-Electrospray Tandem Mass Spectrometry

A rapid and sensitive liquid chromatography/electrospray ionization/tandem mass spectrometry (LC-ESI-MS-MS) method has been developed for the determination of the plant growth regulator paclobutrazol in pear samples. Extraction was performed with methanol by using a high-speed blender Ultra-Turrax,...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2003-07, Vol.51 (15), p.4202-4206
Main Authors: Sancho, Juan V, Pozo, Óscar J, Zamora, Tatiana, Grimalt, Susana, Hernández, Félix
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromatography, High Pressure Liquid
detection limit
Drug Residues - analysis
Food industries
Fruit - chemistry
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
General aspects
ionization
liquid chromatography
mass spectrometry
methanol
Methods of analysis, processing and quality control, regulation, standards
paclobutrazol
pears
plant growth substances
Pyrus - chemistry
Quality Control
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
Triazoles - analysis
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Summary:A rapid and sensitive liquid chromatography/electrospray ionization/tandem mass spectrometry (LC-ESI-MS-MS) method has been developed for the determination of the plant growth regulator paclobutrazol in pear samples. Extraction was performed with methanol by using a high-speed blender Ultra-Turrax, and 10 μL of pear extract was directly injected in the LC-ESI-MS-MS system without any previous sample treatment. The highest sensitivity of the method was achieved under MS-MS conditions obtaining a limit of detection of 0.7 μg/kg and a quantification limit of 5 μg/kg, with a run time of only 5.5 min. Recoveries for paclobutrazol from spiked pear samples at 0.005, 0.05, and 0.5 mg/kg were around 82−102% with relative standard deviations between 2 and 7%. The method was applied to real treated and untreated samples of pears, using quality control samples as a evaluation of the method reliability. Two MS-MS transitions were selected, one for quantification (294 > 70) and the other for confirmation of the analyte (296 > 70). All the experiments were performed in compliance with good laboratory practices. Keywords: Paclobutrazol; pesticides; fruit samples; liquid chromatography; tandem mass spectrometry
ISSN:0021-8561
1520-5118
DOI:10.1021/jf034107s