Determination of Aloesin and Aloeresin A for the Detection of Aloe in Beverages

This work describes a sensitive high-performance liquid chromatography (HPLC) method for the quantification of aloesin and aloeresin A in alcoholic beverages containing aloe as a flavoring agent. The compounds were prepared from Aloe ferox juice. Sephadex LH20 and ion-exchange resin AG1X2 column chr...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2007-05, Vol.55 (9), p.3363-3367
Main Authors: Dell'Agli, Mario, Giavarini, Flavio, Ferraboschi, Patrizia, Galli, Giovanni, Bosisio, Enrica
Format: Article
Language:English
Subjects:
alcoholic beverages
Alcoholic Beverages - analysis
Aloe - chemistry
Aloe ferox
aloeresin A
aloesin
Beverages - analysis
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Chromones - analysis
flavor compounds
flavorings
food analysis
Food Contamination - analysis
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Glucosides - analysis
high performance liquid chromatography
Methods of analysis, processing and quality control, regulation, standards
Plant Leaves - chemistry
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Summary:This work describes a sensitive high-performance liquid chromatography (HPLC) method for the quantification of aloesin and aloeresin A in alcoholic beverages containing aloe as a flavoring agent. The compounds were prepared from Aloe ferox juice. Sephadex LH20 and ion-exchange resin AG1X2 column chromatography were used for aloesin. Aloeresin A was obtained by Sephadex LH20 and silica gel column chromatography followed by purification on Discovery DSC-18 solid-phase extraction tubes. A 98 mg amount of aloesin (>99% purity) and 34 mg of aloeresin A (>98% purity) were recovered from 2.5 g of aloe juice. The HPLC method was validated, and intra- and interday performances were established. In-house validation was carried out by analyzing samples of beverages with and without aloe as a flavoring agent. Keywords: Aloesin; aloeresin A; C-glucosylchromones; aloe juice; hydroalcoholic beverages; Aloe ferox
ISSN:0021-8561
1520-5118
DOI:10.1021/jf070182h