A Novel P106L Mutation in EPSPS and an Unknown Mechanism(s) Act Additively To Confer Resistance to Glyphosate in a South African Lolium rigidum Population

Glyphosate resistance evolution in weeds is a growing problem in world agriculture. Here, we have investigated the mechanism(s) of glyphosate resistance in a Lolium rigidum population (DAG1) from South Africa. Nucleotide sequencing revealed the existence of at least three EPSPS homologues in the L....

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Published in:Journal of agricultural and food chemistry 2011-04, Vol.59 (7), p.3227-3233
Main Authors: Kaundun, Shiv S, Dale, Richard P, Zelaya, Ian A, Dinelli, Giovanni, Marotti, Ilaria, McIndoe, Eddie, Cairns, Andrew
Format: Article
Language:English
Subjects:
3-Phosphoshikimate 1-Carboxyvinyltransferase - genetics
amino acid substitution
Biological and medical sciences
Crop and Animal Protection Chemistry
Food industries
Fundamental and applied biological sciences. Psychology
genome
Genotype
Glycine - analogs & derivatives
Glycine - metabolism
Glycine - pharmacology
Glyphosate
Herbicide Resistance - genetics
Herbicides - metabolism
Herbicides - pharmacology
leucine
Lolium - drug effects
Lolium - enzymology
Lolium - genetics
Lolium rigidum
metabolism
Mutation
point mutation
proline
South Africa
weeds
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Summary:Glyphosate resistance evolution in weeds is a growing problem in world agriculture. Here, we have investigated the mechanism(s) of glyphosate resistance in a Lolium rigidum population (DAG1) from South Africa. Nucleotide sequencing revealed the existence of at least three EPSPS homologues in the L. rigidum genome and identified a novel proline 106 to leucine substitution (P106L) in 52% DAG1 individuals. This mutation conferred a 1.7-fold resistance increase to glyphosate at the whole plant level. Additionally, a 3.1-fold resistance increase, not linked to metabolism or translocation, was estimated between wild-type P106-DAG1 and P106-STDS sensitive plants. Point accepted mutation analysis suggested that other amino acid substitutions at EPSPS position 106 are likely to be found in nature besides the P106/S/A/T/L point mutations reported to date. This study highlights the importance of minor mechanisms acting additively to confer significant levels of resistance to commercial field rates of glyphosate in weed populations subjected to high selection pressure.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf104934j