Bovine Serum Albumin Produces a Synergistic Increase in the Antioxidant Activity of Virgin Olive Oil Phenolic Compounds in Oil-in-Water Emulsions

• Published in: Journal of agricultural and food chemistry 2008-08, Vol.56 (16), p.7076-7081
• Main Authors: Bonoli-Carbognin, Matteo, Cerretani, Lorenzo, Bendini, Alessandra, Almajano, M. Pilar, Gordon, Michael H
• Format: Article
• Language: English
• Subjects: albumin; Antioxidant; antioxidant activity; Antioxidants - pharmacology; Biological and medical sciences; bovine serum albumin; Chemical Changes Induced by Processing/Storage; Drug Synergism; emulsions; Emulsions - chemistry; Fat industries; Food industries; food storage; Fundamental and applied biological sciences. Psychology; lipid peroxidation; Lipid Peroxidation - drug effects; Milk and cheese industries. Ice creams; model food systems; off flavors; oil in water emulsion; Olive Oil; oxidative stability; phenolic compounds; Phenols - pharmacology; Plant Oils - chemistry; Serum Albumin, Bovine - pharmacology; storage quality; storage time; synergism; virgin olive oil; volatile compounds
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf800743r

Virgin olive oil is valued for its flavor, but unacceptable off-flavors may develop on storage in food products containing this oil due to oxidation. The oxidative stability of oil-in-water emulsions containing bovine serum albumin (BSA) and virgin olive oil phenolic compounds was studied. Four oil-in-water emulsions with and without BSA and phenols isolated from virgin olive oil were prepared. These model systems were stored at 60 °C to speed up lipid oxidation. Primary and secondary oxidation products were monitored every three days. Peroxide values and conjugated diene contents were determined as measures of the primary oxidation products. p-Anisidine values and volatile compounds were determined as measures of the secondary oxidation products. This latter determination was carried out by headspace solid-phase microextraction coupled with gas chromatography. Although olive oil phenolic compounds and BSA contributed some antioxidant activity when present as individual additives, the combination of BSA with phenols in an emulsion showed 58−127% synergy, depending on which analytical method was used in the calculation. The emulsion containing phenolic compounds and BSA showed a low level of deterioration after 45 days of storage at 60 °C.