Development of an Enzyme-Linked Immunosorbent Assay for the α-Cyano Pyrethroids Multiresidue in Tai Lake Water

A general and broad class selective competitive enzyme-linked immunosorbent assay (ELISA) for the detection of the α-cyano pyrethroids was developed. One class specific hapten, the 5-(3-benzylphenyl)-5-cyanopentanoic acid (CPBA-BE), was conjugated with BSA as an immunogen. Polyclonal antibodies, PY-...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2009-04, Vol.57 (8), p.3033-3039
Main Authors: Hao, X. L, Kuang, H, Li, Y. L, Yuan, Y, Peng, C. F, Chen, W, Wang, L. B, Xu, C. L
Format: Article
Language:English
Subjects:
alpha-cyano pyrethroids
Analytical Methods
Biological and medical sciences
China
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Food industries
Fresh Water - chemistry
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
insecticide residues
Insecticides - analysis
lakes
multiresidue analysis
Osmolar Concentration
pesticide residues
Pesticide Residues - analysis
pyrethrins
Pyrethrins - analysis
pyrethroid insecticides
surface water
Water Pollutants, Chemical - analysis
water pollution
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Summary:A general and broad class selective competitive enzyme-linked immunosorbent assay (ELISA) for the detection of the α-cyano pyrethroids was developed. One class specific hapten, the 5-(3-benzylphenyl)-5-cyanopentanoic acid (CPBA-BE), was conjugated with BSA as an immunogen. Polyclonal antibodies, PY-antibodies, were generated. The assay with the most selectivity for the family α-cyano pyrethroids such as cyphenothrin was further optimized and tested for tolerance to cosolvent, pH, and ionic strength changes. The IC50 values of the optimized immunoassay were 4.58 μg L−1 for cyphenothrin, 5.62 μg L−1 for fenpropathrin, 7.08 μg L−1 for deltamethrin, 10.72 μg L−1 for cypermethrin, 19.95 μg L−1 for flucythrinate, and 28.18 μg L−1 for esfenvalerate. The cross-reactivities of cyphenothrin, fenpropathrin, deltamethrin, cypermethrin, flucythrinate, and esfenvalerate were 100%, 81.49%, 64.68%, 42.72%, 22.96%, and 16.25%, respectively. The low detection limit (LDL) of this assay was 0.107 μg L−1 for cyphenothrin. This method was used for the detection of the α-cyano pyrethroids in Tai lake water.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf803807b