Biosynthesis of 2-Methylbutyl, 2-Methyl-2-butenyl, and 2-Methylbutanoate Esters in Red Delicious and Granny Smith Apples Using Deuterium-Labeled Substrates

2-Methylbutanoate esters, especially ethyl 2-methylbutanoate, are key contributors to fruit aroma. The biosynthetic origins and interconversions of 2-methylbutyl and 2-methylbutanoate esters in Red Delicious and Granny Smith apples were determined by feeding deuterium-labeled substrates with GC−MS i...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 1996-10, Vol.44 (10), p.3276-3285
Main Authors: Rowan, Daryl D, Lane, Heather P, Allen, John M, Fielder, Simon, Hunt, Martin B
Format: Article
Language:English
Subjects:
ACIDE GRAS VOLATIL
ACIDO GRASO VOLATIL
APPLES
AROMA
Biological and medical sciences
COMPOSE VOLATIL
COMPUESTO VOLATIL
CULTIVARS
ESTER
ESTERES
ESTERS
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
ISOTOPE LABELING
MANZANA
METABOLISM
METABOLISME
METABOLISMO
ODEUR
OLOR
POMME
SMELL
TECHNIQUE DES TRACEURS
TECNICAS DE TRAZADORES
TRACER TECHNIQUES
VARIEDADES
VARIETE
VARIETIES
VOLATILE COMPOUNDS
VOLATILE FATTY ACIDS
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:2-Methylbutanoate esters, especially ethyl 2-methylbutanoate, are key contributors to fruit aroma. The biosynthetic origins and interconversions of 2-methylbutyl and 2-methylbutanoate esters in Red Delicious and Granny Smith apples were determined by feeding deuterium-labeled substrates with GC−MS identification of the deuterated aroma volatiles produced. Deuterium-labeled isoleucine was fed to apple peel, and 2-methylbutanoic-d 3 acid, 2-methylbutanol-d 3, and ethyl 2-methylbutanoate-d 3 were fed as vapor to whole apples. An array of labeled 2-methylbutyl and 2-methylbutanoate esters was produced from each substrate with significant differences in products and product distributions between the two apple cultivars. Novel 2-methyl-(2E)-butenyl esters were identified as biosynthetic products in the aroma of Red Delicious but not Granny Smith apples. Keywords: Biosynthesis; apple; aroma volatiles; 2-methylbutyl; 2-methylbutanoate; 2-methyl-2-butenyl; deuterium
ISSN:0021-8561
1520-5118
DOI:10.1021/jf9508209