On-Line HPLC Photodiode Array Detection and OPA Derivatization for Partial Identification of Small Peptides from White Wine

This paper reports the possibilities of on-line reversed-phase HPLC with photodiode array detection (PDAD) and derivatization with o-phthaldialdehyde (OPA) for identification of small peptides. The methodology was initially applied to a wide range of standard peptides. Spectral parameters (wavelengt...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1997-09, Vol.45 (9), p.3374-3381
Main Authors: Bartolomé, Begoña, Moreno-Arribas, Victoria, Pueyo, Encarnación, Polo, M. Carmen
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fermented food industries
Food industries
Fundamental and applied biological sciences. Psychology
HPLC
IDENTIFICACION
IDENTIFICATION
O-PHTHALDIALDEHYDE
PEPTIDE
PEPTIDES
PEPTIDOS
VIN
VINOS
WINES
Wines and vinegars
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Summary:This paper reports the possibilities of on-line reversed-phase HPLC with photodiode array detection (PDAD) and derivatization with o-phthaldialdehyde (OPA) for identification of small peptides. The methodology was initially applied to a wide range of standard peptides. Spectral parameters (wavelengths of the spectrum maxima, convexity interval, and wavelengths of the second-derivative spectrum maxima) obtained by PDAD allow identification of the aromatic amino acid residues (tyrosine, tryptophan, and phenylalanine) contained in the peptides as well as identification of other compounds from wine that can coelute with peptides (i.e. phenolic compounds). Postcolumn OPA derivatization allows confirmation of the peptidic nature of chromatographic peaks. The methodology has been successfully applied to peptide fractions (
ISSN:0021-8561
1520-5118
DOI:10.1021/jf9700844