Anthocyanins as Antioxidants on Human Low-Density Lipoprotein and Lecithin−Liposome Systems

The antioxidant activity of several anthocyanins was tested in vitro on human low-density lipoproteins (LDL) and on a lecithin−liposome system. Samples were incubated at 37 °C, and the extent of oxidation was measured by determining the formation of conjugated diene and hexanal. The inhibition of ox...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1997-09, Vol.45 (9), p.3362-3367
Main Authors: Satué-Gracia, M. Teresa, Heinonen, Marina, Frankel, Edwin N
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
ANTHOCYANE
ANTHOCYANINS
ANTIOXIDANTES
ANTIOXIDANTS
ANTIOXYDANT
ANTOCIANINAS
Aromatic and heterocyclic compounds
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Heterocyclic compounds, pigments
LIPOPROTEINAS
LIPOPROTEINE
LIPOPROTEINS
NATURAL ANTIOXIDATNS
Other biological molecules
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Summary:The antioxidant activity of several anthocyanins was tested in vitro on human low-density lipoproteins (LDL) and on a lecithin−liposome system. Samples were incubated at 37 °C, and the extent of oxidation was measured by determining the formation of conjugated diene and hexanal. The inhibition of oxidation increased with concentration of the antioxidant. In the LDL system, when the oxidation was catalyzed with 10 μM copper, malvidin was the best oxidation inhibitor, followed by delphinidin, cyanidin, and pelargonin. When the oxidation was catalyzed with 80 μM copper, the order of antioxidant activity changed and decreased in the following order at all concentrations tested:  delphinidin, cyanidin, malvidin, and pelargonin. In the liposome system, catalyzed with either 3 or 10 μM copper, malvidin was the best inhibitor of both conjugated diene and hexanal formation. At 3 μM copper, delphinidin, cyanidin, and pelargonin showed prooxidant activity. At 10 μM copper, pelargonin followed malvidin in antioxidant potency, and cyanidin and delphinidin were prooxidants. Several antioxidant mechanisms may explain the effect of anthocyanins, including hydrogen donation, metal chelation, and protein binding. Keywords: Anthocyanin; antioxidant evaluation; LDL oxidation; liposome oxidation; natural antioxidants
ISSN:0021-8561
1520-5118
DOI:10.1021/jf970234a