Microwave-Assisted Extraction of Taxanes from Taxus Biomass

A method is presented for the extraction of the taxane class of natural products, including paclitaxel, from Taxus needles using microwave-assisted extraction (MAE). Various temperatures, times, and organic solvents for the MAE procedure were investigated to optimize the efficiency of the extraction...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1997-12, Vol.45 (12), p.4691-4696
Main Authors: Mattina, M. J. Incorvia, Berger, W. A. Iannucci, Denson, C. L
Format: Article
Language:English
Subjects:
Biological and medical sciences
BIOMASA
BIOMASS
BIOMASSE
CONIFERALE
CONIFERALES
DRUG PLANTS
EXTRACCION
EXTRACTION
FEUILLE
Fundamental and applied biological sciences. Psychology
General pharmacology
HOJAS
LEAVES
Medical sciences
Methods and techniques. Experimental designs. Plant protection equipments
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Phytopathology. Animal pests. Plant and forest protection
PLANTAS MEDICINALES
PLANTE MEDICINALE
TAXUS
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Summary:A method is presented for the extraction of the taxane class of natural products, including paclitaxel, from Taxus needles using microwave-assisted extraction (MAE). Various temperatures, times, and organic solvents for the MAE procedure were investigated to optimize the efficiency of the extraction. The effects of biomass to solvent ratio and the water content of the system on taxane recovery were also determined. Under appropriate MAE conditions using 95% ethanol, an extract of the needles was obtained which was equivalent to that produced by a conventional extraction method. The conventional method consisted of an overnight shake of 5 g of needles in 100 mL of methanol at ambient temperature. Thus, with optimized parameter settings, MAE was found to reduce considerably both extraction time and solvent consumption, while maintaining qualitative and quantitative taxane recovery relative to traditional solid/liquid extraction methods. Keywords: Taxanes; paclitaxel; microwave-assisted extraction; Taxus needles
ISSN:0021-8561
1520-5118
DOI:10.1021/jf970454o