Inhibitors of Arachidonate Lipoxygenase from Defatted Perilla Seed

The effects of phenolic compounds isolated from defatted perilla (Perilla frutescens var. japonica Hara) seed on arachidonate 12-lipoxygenase (12-LO) and 5-lipoxygenase (5-LO) activities were investigated using rat platelets and polymorphonuclear leukocytes. The ethyl acetate layer obtained by parti...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1998-03, Vol.46 (3), p.862-865
Main Authors: Yamamoto, Hiroyo, Sakakibara, Jinsaku, Nagatsu, Akito, Sekiya, Keizo
Format: Article
Language:English
Subjects:
ARACHIDONATE 12-LIPOXYGEANSE
ARACHIDONATE 5-LIPOXYGENASE
ARACHIDONIC ACID
ARACHIDONIC ACID METABOLISM
Biological and medical sciences
ENZYME INHIBITORS
ENZYMIC ACTIVITY
General pharmacology
INDUSTRIAL WASTES
LEUKOCYTES
LIPID METABOLISM
Medical sciences
MEDICINAL PROPERTIES
OILSEEDS
OXIDOREDUCTASES
PERILLA FRUTESCENS
PERILLA FRUTESCENS VAR. JAPONICA
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
PHENOLIC COMPOUNDS
PLANT EXTRACTS
PLATELETS
WASTE UTILIZATION
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Summary:The effects of phenolic compounds isolated from defatted perilla (Perilla frutescens var. japonica Hara) seed on arachidonate 12-lipoxygenase (12-LO) and 5-lipoxygenase (5-LO) activities were investigated using rat platelets and polymorphonuclear leukocytes. The ethyl acetate layer obtained by partitioning the ethanolic extract of defatted perilla seed proved to have significant inhibitory activity against lipoxygenases. Potent inhibitory principles in the ethyl acetate layer were isolated and identified as luteolin, chrysoeriol, and rosemarinic acid and its methyl ester. The most effective inhibitor of lipoxygenases was luteolin (IC50 = 20 and 102 nM for 12-LO and 5-LO, respectively). These results indicate that defatted perilla seed, now an industrial waste product, may be developed into a useful source of an agent that prevents allergic hyper-reactivity and inflammatory responses due to its antilipoxygenase activity. Keywords: Arachidonic acid; lipoxygenase; perilla seed; luteolin
ISSN:0021-8561
1520-5118
DOI:10.1021/jf970520m