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Variation in Ferulic Acid Concentration among Diverse Barley Cultivars Measured by HPLC and Microspectrophotometry
Ferulic acid (FA) is a low molecular weight phenolic acid that is a common component of the outer layers of cereal grains. It has been implicated in resistance to both insect and fungal pathogens. The FA concentrations of 18 cultivars of 2-row and 6-row barleys, grown in 2 locations, were quantified...
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| Published in: | Journal of agricultural and food chemistry 1998-04, Vol.46 (4), p.1350-1354 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a412t-2cc0c176df325632f9426b1e5712ef8d0d689921b77dc04bf318f66fd7d308a03 |
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| cites | cdi_FETCH-LOGICAL-a412t-2cc0c176df325632f9426b1e5712ef8d0d689921b77dc04bf318f66fd7d308a03 |
| container_end_page | 1354 |
| container_issue | 4 |
| container_start_page | 1350 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 46 |
| creator | Zupfer, J. M Churchill, K. E Rasmusson, D. C Fulcher, R. G |
| description | Ferulic acid (FA) is a low molecular weight phenolic acid that is a common component of the outer layers of cereal grains. It has been implicated in resistance to both insect and fungal pathogens. The FA concentrations of 18 cultivars of 2-row and 6-row barleys, grown in 2 locations, were quantified by HPLC analysis following acid hydrolysis. The FA concentrations ranged from 365 to 605 μg/g dry weight. The concentration of FA varied significantly among the different cultivars of barley. A genetic basis for FA levels is inferred by the similar ranking of the cultivars in both locations. FA levels were also measured by the absorbance at 340 nm of the acid-hydrolyzed extract, and in situ detection of fluorescence intensity of the ground grain using a UMSP80 microspectrofluorimeter. Both of these methods are more rapid than HPLC and may allow the practical screening of FA concentrations in numerous barley lines. Keywords: Ferulic acid; phenolics; fluorescence; UV absorption; barley |
| doi_str_mv | 10.1021/jf9708103 |
| format | article |
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M ; Churchill, K. E ; Rasmusson, D. C ; Fulcher, R. G</creator><creatorcontrib>Zupfer, J. M ; Churchill, K. E ; Rasmusson, D. C ; Fulcher, R. G</creatorcontrib><description>Ferulic acid (FA) is a low molecular weight phenolic acid that is a common component of the outer layers of cereal grains. It has been implicated in resistance to both insect and fungal pathogens. The FA concentrations of 18 cultivars of 2-row and 6-row barleys, grown in 2 locations, were quantified by HPLC analysis following acid hydrolysis. The FA concentrations ranged from 365 to 605 μg/g dry weight. The concentration of FA varied significantly among the different cultivars of barley. A genetic basis for FA levels is inferred by the similar ranking of the cultivars in both locations. FA levels were also measured by the absorbance at 340 nm of the acid-hydrolyzed extract, and in situ detection of fluorescence intensity of the ground grain using a UMSP80 microspectrofluorimeter. Both of these methods are more rapid than HPLC and may allow the practical screening of FA concentrations in numerous barley lines. Keywords: Ferulic acid; phenolics; fluorescence; UV absorption; barley</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf9708103</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>ABSORBANCE ; Agronomy. Soil science and plant productions ; ANALYTICAL METHODS ; BARLEY ; Biological and medical sciences ; CEREALS ; DETECTION ; FERULIC ACID ; FLUORESCENCE ; Fundamental and applied biological sciences. Psychology ; Generalities ; Genetics and breeding of economic plants ; GEOGRAPHICAL DISTRIBUTION ; GRAIN ; HORDEUM VULGARE ; HPLC ; MEASUREMENT ; Pest resistance ; PROVENANCE ; QUANTITATIVE ANALYSIS ; RAPID METHODS ; SCREENING ; SPECTROMETRY ; TESTING ; Varietal selection. 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M</creatorcontrib><creatorcontrib>Churchill, K. E</creatorcontrib><creatorcontrib>Rasmusson, D. C</creatorcontrib><creatorcontrib>Fulcher, R. G</creatorcontrib><title>Variation in Ferulic Acid Concentration among Diverse Barley Cultivars Measured by HPLC and Microspectrophotometry</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Ferulic acid (FA) is a low molecular weight phenolic acid that is a common component of the outer layers of cereal grains. It has been implicated in resistance to both insect and fungal pathogens. The FA concentrations of 18 cultivars of 2-row and 6-row barleys, grown in 2 locations, were quantified by HPLC analysis following acid hydrolysis. The FA concentrations ranged from 365 to 605 μg/g dry weight. The concentration of FA varied significantly among the different cultivars of barley. A genetic basis for FA levels is inferred by the similar ranking of the cultivars in both locations. FA levels were also measured by the absorbance at 340 nm of the acid-hydrolyzed extract, and in situ detection of fluorescence intensity of the ground grain using a UMSP80 microspectrofluorimeter. Both of these methods are more rapid than HPLC and may allow the practical screening of FA concentrations in numerous barley lines. Keywords: Ferulic acid; phenolics; fluorescence; UV absorption; barley</description><subject>ABSORBANCE</subject><subject>Agronomy. Soil science and plant productions</subject><subject>ANALYTICAL METHODS</subject><subject>BARLEY</subject><subject>Biological and medical sciences</subject><subject>CEREALS</subject><subject>DETECTION</subject><subject>FERULIC ACID</subject><subject>FLUORESCENCE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities</subject><subject>Genetics and breeding of economic plants</subject><subject>GEOGRAPHICAL DISTRIBUTION</subject><subject>GRAIN</subject><subject>HORDEUM VULGARE</subject><subject>HPLC</subject><subject>MEASUREMENT</subject><subject>Pest resistance</subject><subject>PROVENANCE</subject><subject>QUANTITATIVE ANALYSIS</subject><subject>RAPID METHODS</subject><subject>SCREENING</subject><subject>SPECTROMETRY</subject><subject>TESTING</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><subject>VARIETIES</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNptkEtv1DAUhSMEEkNhwQ9A8gIWLALXTmI7yxIoRZpCUR9b644fxUMmHl1nKubfkyhoVqy8-L57fHSK4jWHDxwE_7gNrQLNoXpSrHgjoGw410-LFUyw1I3kz4sXOW8BQDcKVgXdI0UcYxpYHNiFp0MfLTu30bEuDdYPIy0Ud2l4YJ_jo6fs2Sek3h9Zd-jH-IiU2ZXHfCDv2ObILq_XHcPBsatoKeW9tyOl_a80pp0f6fiyeBawz_7Vv_esuLv4cttdlusfX7915-sSay7GUlgLlivpQiUaWYnQ1kJuuG8UFz5oB07qthV8o5SzUG9CxXWQMjjlKtAI1VnxfsmdS2Tywewp7pCOhoOZxzKnsSb37eLuMVvsA-FgYz4dCFFDrdWklYsW8-j_nDDSbyNVpRpze31j2p_3AuT31swV3ix-wGTwgabIuxvezr-2DZ_z3i0cbTbbdKBhGuQ_9f4CDQ-NUA</recordid><startdate>19980401</startdate><enddate>19980401</enddate><creator>Zupfer, J. M</creator><creator>Churchill, K. E</creator><creator>Rasmusson, D. C</creator><creator>Fulcher, R. 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Soil science and plant productions</topic><topic>ANALYTICAL METHODS</topic><topic>BARLEY</topic><topic>Biological and medical sciences</topic><topic>CEREALS</topic><topic>DETECTION</topic><topic>FERULIC ACID</topic><topic>FLUORESCENCE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities</topic><topic>Genetics and breeding of economic plants</topic><topic>GEOGRAPHICAL DISTRIBUTION</topic><topic>GRAIN</topic><topic>HORDEUM VULGARE</topic><topic>HPLC</topic><topic>MEASUREMENT</topic><topic>Pest resistance</topic><topic>PROVENANCE</topic><topic>QUANTITATIVE ANALYSIS</topic><topic>RAPID METHODS</topic><topic>SCREENING</topic><topic>SPECTROMETRY</topic><topic>TESTING</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>VARIETIES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zupfer, J. M</creatorcontrib><creatorcontrib>Churchill, K. E</creatorcontrib><creatorcontrib>Rasmusson, D. C</creatorcontrib><creatorcontrib>Fulcher, R. G</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zupfer, J. M</au><au>Churchill, K. E</au><au>Rasmusson, D. C</au><au>Fulcher, R. G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variation in Ferulic Acid Concentration among Diverse Barley Cultivars Measured by HPLC and Microspectrophotometry</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>1998-04-01</date><risdate>1998</risdate><volume>46</volume><issue>4</issue><spage>1350</spage><epage>1354</epage><pages>1350-1354</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Ferulic acid (FA) is a low molecular weight phenolic acid that is a common component of the outer layers of cereal grains. It has been implicated in resistance to both insect and fungal pathogens. The FA concentrations of 18 cultivars of 2-row and 6-row barleys, grown in 2 locations, were quantified by HPLC analysis following acid hydrolysis. The FA concentrations ranged from 365 to 605 μg/g dry weight. The concentration of FA varied significantly among the different cultivars of barley. A genetic basis for FA levels is inferred by the similar ranking of the cultivars in both locations. FA levels were also measured by the absorbance at 340 nm of the acid-hydrolyzed extract, and in situ detection of fluorescence intensity of the ground grain using a UMSP80 microspectrofluorimeter. Both of these methods are more rapid than HPLC and may allow the practical screening of FA concentrations in numerous barley lines. Keywords: Ferulic acid; phenolics; fluorescence; UV absorption; barley</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/jf9708103</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 1998-04, Vol.46 (4), p.1350-1354 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_crossref_primary_10_1021_jf9708103 |
| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | ABSORBANCE Agronomy. Soil science and plant productions ANALYTICAL METHODS BARLEY Biological and medical sciences CEREALS DETECTION FERULIC ACID FLUORESCENCE Fundamental and applied biological sciences. Psychology Generalities Genetics and breeding of economic plants GEOGRAPHICAL DISTRIBUTION GRAIN HORDEUM VULGARE HPLC MEASUREMENT Pest resistance PROVENANCE QUANTITATIVE ANALYSIS RAPID METHODS SCREENING SPECTROMETRY TESTING Varietal selection. Specialized plant breeding, plant breeding aims VARIETIES |
| title | Variation in Ferulic Acid Concentration among Diverse Barley Cultivars Measured by HPLC and Microspectrophotometry |
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