Comparison of a Time-Resolved Fluorescence Immunoassay and an Enzyme-Linked Immunosorbent Assay for the Analysis of Atrazine in Water

Immunoassays for atrazine based on a time-resolved fluorescent label and an enzyme label were optimized and utilized to measure atrazine in water. The time-resolved fluorescent immunoassay (TRFIA) was based on a polyclonal antibody and a europium label, whereas the enzyme-linked immunosorbent assay...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1998-08, Vol.46 (8), p.3353-3358
Main Authors: Reimer, Gerry J, Gee, Shirley J, Hammock, Bruce D
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
AGUA
Analysis methods
ANALYTICAL METHODS
Applied sciences
ATRAZINA
ATRAZINE
Biological and medical sciences
BRITISH COLUMBIA
CALIFORNIA
CALIFORNIE
CHEMICAL COMPOSITION
COLOMBIE BRITANNIQUE
COLUMBIA BRITANICA
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CONTAMINANTES
CONTAMINANTS
COURS D'EAU
CURSOS DE AGUA
DISTRIBUCION GEOGRAFICA
DISTRIBUTION GEOGRAPHIQUE
EAU
ELISA
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
GEOGRAPHICAL DISTRIBUTION
IMMUNOLOGICAL TECHNIQUES
MARISMA
MARSHES
Natural water pollution
POLLUANT
POLLUTANTS
Pollution
PRAIRIE MARECAGEUSE
RIVERS
Soil and water pollution
Soil science
TECHNIQUE ANALYTIQUE
TECHNIQUE IMMUNOLOGIQUE
TECNICAS ANALITICAS
TECNICAS INMUNOLOGICAS
TEST ELISA
WATER
Water treatment and pollution
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Summary:Immunoassays for atrazine based on a time-resolved fluorescent label and an enzyme label were optimized and utilized to measure atrazine in water. The time-resolved fluorescent immunoassay (TRFIA) was based on a polyclonal antibody and a europium label, whereas the enzyme-linked immunosorbent assay (ELISA) utilized a monoclonal antibody and horseradish peroxidase as the label. Detection limits and IC50 values calculated from standard curves were 0.05 ± 0.03 and 0.17 ± 0.08 ng/mL (n = 8) for the TRFIA, respectively, and 0.05 ± 0.04 and 0.3 ± 0.2 ng/mL (n = 17) for the ELISA, respectively. Four different environmental water samples were fortified at various levels of atrazine. When these samples were analyzed, the % RSD for replicate fluorescence or absorbance readings was small (5 and 6%, respectively). The average accuracies for the TRFIA and ELISA were 1.4 ± 0.42 (n = 13) and 1.0 ± 0.38 (n = 13), respectively, reflecting the slight bias of the TRFIA. TRFIA offers an advantage over ELISA in that the fluorescent label is less susceptible to interferences that inhibit enzyme activity and reagents may be more stable than enzyme reagents. Keywords: Atrazine; triazine; herbicide; time-resolved fluorescence; immunoassay; enzyme-linked immunosorbent assay
ISSN:0021-8561
1520-5118
DOI:10.1021/jf970965a