Further Studies on the Analysis of DSP Toxin Profiles in Galician Mussels

Further studies on mussel samples from Galicia, Spain, have revealed the presence of okadaic acid (OA), dinophysistoxin 2 (DTX2), and the fatty acid acyl esters of both of these toxins as the “DTX3” complex. Measurements were performed with an improved in situ method for the formation of 9-anthryldi...

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Published in:Journal of agricultural and food chemistry 1999-02, Vol.47 (2), p.618-621
Main Authors: Comesaña Losada, M, Leão, J. M, Gago-Martínez, A, Rodríguez Vázquez, J. A, Quilliam, M. A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bivalvia - chemistry
Chromatography, Liquid
diarrhetic shellfish poisoning
dinophysistoxin
Fish and seafood industries
food analysis
Food industries
Food toxicology
foodborne illness
Fundamental and applied biological sciences. Psychology
galicaia, spain
Mass Spectrometry
Medical sciences
mussels
okadaic acid
Okadaic Acid - analysis
Pyrans - analysis
Spectrometry, Fluorescence
Toxicology
toxins
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Summary:Further studies on mussel samples from Galicia, Spain, have revealed the presence of okadaic acid (OA), dinophysistoxin 2 (DTX2), and the fatty acid acyl esters of both of these toxins as the “DTX3” complex. Measurements were performed with an improved in situ method for the formation of 9-anthryldiazomethane (ADAM) derivatives followed by liquid chromatography with fluorescence detection. Base hydrolysis of DTX3 toxins gave free OA and DTX2, which were determined following ADAM derivatization. Results were confirmed by liquid chromatography/mass spectrometry analyses, and in most of the samples, free DTX2 was the most abundant toxin. However, the OA/DTX2 ratio in the DTX3 conjugated form was different, with OA being the most abundant in all cases. This difference could be due to different rates of metabolism of OA and DTX2 to the acyl esters or due to contamination of the shellfish by the two toxins at different points in time, resulting in less acyl ester formation for one toxin versus the other. The second possibility would be reasonable if two different source organisms were producing the toxins. Keywords: DSP toxins; okadaic acid; DTX2; DTX3; liquid chromatography; fluorescence; mass spectrometry
ISSN:0021-8561
1520-5118
DOI:10.1021/jf971043a