Improved Enzyme-Linked Immunosorbent Assay To Detect Didecyldimethylammonium Chloride, a Quaternary Ammonium Compound

Didecyldimethylammonium chloride (DDAC) is a commonly used wood protectant in Canada and must be monitored due to environmental concerns. Three new DDAC-like immunogens were synthesized and tested for their ability to produce antibodies that complex DDAC. The polyclonal antisera from rabbits immuniz...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1998-11, Vol.46 (11), p.4779-4786
Main Authors: Bull, Jennifer P, Serreqi, Alessio N, Gamboa, Hector R, Breuil, Colette
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Analysis methods
ANTIGENE
ANTIGENOS
ANTIGENS
Applied sciences
Biological and medical sciences
CANADA
COMPETITIVE ELISA
COMPOSE D'AMMONIUM QUATERNAIRE
COMPUESTOS AMONICOS CUATERNARIOS
CONSERVANTES DE LA MADERA
CONTROLE CONTINU
DETECTION
ELISA
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
HAPTENE
HAPTENOS
HAPTENS
MONITORING
Natural water pollution
Pollution
POLYCLONAL ANTIBODIES
PRODUIT DE PRESERVATION DU BOIS
QUATERNARY AMMONIUM COMPOUNDS
Soil and water pollution
Soil science
SYNTHESIS
TEST ELISA
VIGILANCIA
Water treatment and pollution
WOOD PRESERVATIVES
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Summary:Didecyldimethylammonium chloride (DDAC) is a commonly used wood protectant in Canada and must be monitored due to environmental concerns. Three new DDAC-like immunogens were synthesized and tested for their ability to produce antibodies that complex DDAC. The polyclonal antisera from rabbits immunized with the new hapten conjugates were compared by hapten homologous indirect competitive enzyme-linked immunosorbent assay (CELISA). The resulting antisera recognized DDAC to different degrees. CELISA based on anti-DDAC 3, 2, and 1 antisera had IC50s of 0.05, 16.0, and 17.2 μg/mL, respectively. All the antisera were more sensitive to DDAC than a previously reported polyclonal antiserum. The most sensitive and selective antiserum was produced from the hapten that most closely mimicked DDAC. The selectivity of the three immunoassays for compounds representing DDAC's different epitopes was tested. Each immunoassay exhibited the highest selectivity with compounds that most closely resembled the hapten. The effect of solvents and detergents was determined for the most sensitive immunoassay:  CELISA based on anti-DDAC 3 antiserum. Recovery experiments were also carried out with this assay. This immunoassay (IC50 50 ppb) holds promise for the analysis of environmental samples since the regulatory discharge limit is 700 ppb. Keywords: Didecyldimethylammonium chloride; quaternary ammonium compounds; ELISA; immunoassay
ISSN:0021-8561
1520-5118
DOI:10.1021/jf980047s