Methodology for Identification of Phenolic Acids in Complex Phenolic Mixtures by High-Resolution Two-Dimensional Nuclear Magnetic Resonance. Application to Methanolic Extracts of Two Oregano Species

Spectroscopic methodology in analyzing two-dimensional (2D) NMR spectra of a mixture of several phenolic compounds that occur in natural products is described. Particular emphasis has been given to the determination of scalar coupling connectivities by homonuclear 2D correlated spectroscopy (COSY),...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1998-10, Vol.46 (10), p.4185-4192
Main Authors: Gerothanassis, I. P, Exarchou, V, Lagouri, V, Troganis, A, Tsimidou, M, Boskou, D
Format: Article
Language:English
Subjects:
ACIDE PHENOLIQUE
ACIDOS FENOLICOS
ANTIOXIDANTES
ANTIOXIDANTS
ANTIOXYDANT
Biological and medical sciences
Chemical constitution
ESPECTROMETRIA
ESPECTROSCOPIA RMN
EXTRACTOS VEGETALES
EXTRAIT D'ORIGINE VEGETALE
Fundamental and applied biological sciences. Psychology
General pharmacology
IDENTIFICACION
IDENTIFICATION
Medical sciences
NMR SPECTROSCOPY
ORIGANUM
ORIGANUM ONITES
ORIGANUM VULGARE
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
PHENOLIC ACIDS
PLANT EXTRACTS
Plant physiology and development
SPECTRAL ANALYSIS
SPECTROMETRIE
SPECTROMETRY
SPECTROSCOPIE RMN
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Summary:Spectroscopic methodology in analyzing two-dimensional (2D) NMR spectra of a mixture of several phenolic compounds that occur in natural products is described. Particular emphasis has been given to the determination of scalar coupling connectivities by homonuclear 2D correlated spectroscopy (COSY), remote intraresidue connectivities by totally correlated spectroscopy (TOCSY), and spatially close but uncoupled 1H nuclei by homonuclear 2D nuclear Overhauser effect spectroscopy (NOESY/ROESY). Preliminary data to identify phenolic acids in the methanolic extracts from two oregano plants are also reported. Keywords: Oregano; phenolic acids; 1H NMR; COSY; TOCSY; NOESY/ROESY
ISSN:0021-8561
1520-5118
DOI:10.1021/jf9804591