Media Evaluation for the Production of Microbial Enzymes

This study compares the performances of animal-based byproducts with other complex nutrients. Productions of α-amylase by Bacillus subtilis, glucoamylase by Aspergillus niger, endocellulase, exocellulase, and xylanase by Trichoderma viride, protease by B. subtilis, and B. licheniformis were evaluate...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1998-11, Vol.46 (11), p.4775-4778
Main Authors: Lee, Byungtae, Pometto, Anthony L, Demirci, Ali, Hinz, Paul N
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ALFA AMILASA
ALPHA AMYLASE
ANIMAL-BASED BYPRODUCTS
ASPERGILLUS NIGER
BACILLUS LICHENIFORMIS
BACILLUS SUBTILIS
Biological and medical sciences
BIOLOGICAL PRODUCTION
Biotechnology
BYPRODUCTS
CELLULASE
CELULASA
CULTURE MEDIA
ENZIMAS
ENZYME
ENZYMES
ENZYMIC ACTIVITY
FERMENTACION
FERMENTATION
Fundamental and applied biological sciences. Psychology
GLUCAN 1,4-ALPHA-GLUCOSIDASE
MEDIO DE CULTIVO
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
MICROORGANISME
MICROORGANISMOS
MICROORGANISMS
MILIEU DE CULTURE
NUTRIENT SOURCES
NUTRIENTES
NUTRIENTS
PRODUCCION BIOLOGICA
PRODUCTION BIOLOGIQUE
PROTEASAS
PROTEASE
PROTEASES
SOUS PRODUIT
SUBPRODUCTOS
SUBSTANCE NUTRITIVE
TRICHODERMA VIRIDE
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Summary:This study compares the performances of animal-based byproducts with other complex nutrients. Productions of α-amylase by Bacillus subtilis, glucoamylase by Aspergillus niger, endocellulase, exocellulase, and xylanase by Trichoderma viride, protease by B. subtilis, and B. licheniformis were evaluated in shake-flask fermentation. Culture broths were analyzed daily for enzyme activity for up to 9 days. Significant increases in α-amylase, cellulase, and xylanase activities were observed. Data strongly suggest that many of these byproducts can be used to replace the more expensive complex nutrients in industrial enzyme production with a corresponding increase in enzyme production. Keywords: Enzyme production; α-amylase; glucoamylase; endocellulase; exocellulase; xylanase; protease
ISSN:0021-8561
1520-5118
DOI:10.1021/jf980476q