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Rapid Purification of Fumonisins and Their Hydrolysis Products with Solid-Phase Extraction Columns

Fumonisins B3 and B4 (FB3 and FB4) were recovered from the 50:50 acetonitrile/water extract of corn cultures of a strain of Fusarium moniliforme that does not make FB1 or FB2 by stirring the extract with IRA-68, a weak anion-exchange resin. The fumonisins were desorbed with 5% acetic acid in the sam...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1999-06, Vol.47 (6), p.2344-2349
Main Authors: Poling, Stephen M, Plattner, Ronald D
Format: Article
Language:English
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Summary:Fumonisins B3 and B4 (FB3 and FB4) were recovered from the 50:50 acetonitrile/water extract of corn cultures of a strain of Fusarium moniliforme that does not make FB1 or FB2 by stirring the extract with IRA-68, a weak anion-exchange resin. The fumonisins were desorbed with 5% acetic acid in the same solvent. After dilution with water, the desorbed fumonisins were separated into FB3 (FB3 and FA3) and FB4 (FB4, FC4, and FA4) fractions with a tC18 solid-phase extraction (SPE) cartridge. The FB3 fraction was then separated into FB3 and FA3 by using an NH2 SPE cartridge and eluting with 5% acetic acid and increasing amounts of acetonitrile in water. Finally, FB1 and FA3 were hydrolyzed with calcium hydroxide. After recovery from the reaction mixture using a tC18 cartridge, the hydrolyzed and partially hydrolyzed analogues were separated and the unreacted fumonisins recovered by using an NH2 cartridge, initially in the normal-phase mode with increasing amounts of water in acetonitrile and then in the reversed-phase mode after the addition of 5% acetic acid to the solvent and eluting in the reverse order. Keywords: Fumonisins; Fusarium moniliforme; corn; hydrolysis; isolation
ISSN:0021-8561
1520-5118
DOI:10.1021/jf981109h