Saccharomyces c erevisiae Mannoproteins That Protect Wine from Protein Haze:  Evaluation of Extraction Methods and Immunolocalization

• Published in: Journal of agricultural and food chemistry 2000-04, Vol.48 (4), p.1086-1095
• Main Authors: Dupin, Isabelle V. S, Stockdale, Vanessa J, Williams, Patrick J, Jones, Graham P, Markides, Andrew J, Waters, Elizabeth J
• Format: Article
• Language: English
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf9905020

Yeast-derived haze-protective mannoprotein material (HPM) offers protection to white wines from commercially unacceptable turbidities. HPM extraction methods have been evaluated using three winemaking strains of Saccharomyces cerevisiae. Digestion with Zymolyase of cells pretreated with DTE and EDTA gave the greatest yields of active material. Heat treatment of cells with SDS also released active material but the quantities were low. Treatment of the cells in an autoclave or with a French pressure device was less effective. A detailed study was conducted on the strain Maurivin PDM. SDS was not necessary to extract HPM from PDM; boiling the cells for 5 min in Tris buffer was sufficient. HPM could also be extracted with EDTA during the pretreatment of the cells prior to Zymolyase digestion. The data suggest that HPM was noncovalently linked to other cell wall components and loosely associated with the cell wall. An immunological investigation showed that a specific mannoprotein with haze-protective activity, HPF1, was located primarily on the outermost and innermost layers of the cell wall. Keywords: Yeast cell wall, mannoprotein extraction, haze-protective material, SDS, Zymolyase, EDTA, DTE, Saccharomyces cerevisiae, electron microscopy, immuno-gold labeling