Use of Phosphonic Acid as a Generic Hapten in the Production of Broad Specificity Anti-Organophosphate Pesticide Antibody

Phosphonic acid (trans-4-phosphono-2-butenic acid; TPB) was used as a generic hapten in order to generate broad specificity antibodies against a group of organophosphorus pesticides. The polyclonal antiserum showed, in an indirect enzyme-linked immunosorbent assay (ELISA) format, preferential bindin...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2000-06, Vol.48 (6), p.2228-2233
Main Authors: Alcocer, M. J. C, Dillon, P. P, Manning, B. M, Doyen, C, Lee, H. A, Daly, S. J, O'Kennedy, R, Morgan, M. R. A
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibody Specificity
Biological and medical sciences
Biosensing Techniques
Enzyme-Linked Immunosorbent Assay - methods
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Haptens
Insecticides - analysis
Insecticides - immunology
Male
Methods of analysis, processing and quality control, regulation, standards
Organophosphonates - immunology
Rabbits
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Phosphonic acid (trans-4-phosphono-2-butenic acid; TPB) was used as a generic hapten in order to generate broad specificity antibodies against a group of organophosphorus pesticides. The polyclonal antiserum showed, in an indirect enzyme-linked immunosorbent assay (ELISA) format, preferential binding toward pesticides containing unsaturated diethyl-phosphate functionalities rather than the equivalent thiophosphate or dimethyl structures. The level of detection in the ELISA using a heterologous system was investigated and showed a 20-fold improvement when a conjugate for which the antibody had lower affinity was immobilized on the plate. Biosensor assays using parathion as a standard indicated that the antibody had a relatively high dissociation rate, and reproducible cycles of regeneration were achieved. The potential for using TPB as a generic hapten is discussed. Keywords: Organophosphorus pesticides; phosphonate; broad specificity antibody; ELISA; biosensor; immunoassay
ISSN:0021-8561
1520-5118
DOI:10.1021/jf990691m