Structure Determination of Trialanine in Water Using Polarization Sensitive Two-Dimensional Vibrational Spectroscopy

Using polarization sensitive two-dimensional (2D) vibrational spectroscopy on the amide I mode, the central backbone structure of trialanine in aqueous solution is investigated. We exploit the polarization sensitivity of the 2D pump−probe signal to reveal the cross-peak structure hidden under the st...

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Bibliographic Details
Published in:The journal of physical chemistry. B 2000-11, Vol.104 (47), p.11316-11320
Main Authors: Woutersen, S, Hamm, P
Format: Article
Language:English
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Summary:Using polarization sensitive two-dimensional (2D) vibrational spectroscopy on the amide I mode, the central backbone structure of trialanine in aqueous solution is investigated. We exploit the polarization sensitivity of the 2D pump−probe signal to reveal the cross-peak structure hidden under the strong diagonal peaks. The dihedral angles φ and ψ characterizing the peptide backbone structure are derived directly from the cross-peak intensity and anisotropy, demonstrating the potential of 2D spectroscopy as a tool for peptide structure elucidation.
ISSN:1520-6106
1520-5207
DOI:10.1021/jp001546a