Deep-UV Laser-Based Fluorescence Lifetime Imaging Microscopy of Single Molecules

Deep ultraviolet (deep-UV) laser-based fluorescence lifetime imaging microscopy of single dye molecules adsorbed on quartz glass surfaces is presented. The instrumental setup uses a mode-locked diode-pumped picosecond laser emitting at 266 nm with a repetition rate of 40 MHz. Applying the time-resol...

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Bibliographic Details
Published in:The journal of physical chemistry. B 2004-06, Vol.108 (24), p.8324-8329
Main Authors: Li, Qiang, Ruckstuhl, Thomas, Seeger, Stefan
Format: Article
Language:English
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Summary:Deep ultraviolet (deep-UV) laser-based fluorescence lifetime imaging microscopy of single dye molecules adsorbed on quartz glass surfaces is presented. The instrumental setup uses a mode-locked diode-pumped picosecond laser emitting at 266 nm with a repetition rate of 40 MHz. Applying the time-resolved single-photon counting method, we investigated the laser dye 2,2‘ ‘‘-dimethyl-p-quaterphenyl and obtained the fluorescence intensity and fluorescence lifetime images of individual adsorbed molecules. The described setup is also well-suited for biological applications for ultrasensitive detection of intrinsic fluorescence after UV excitation.
ISSN:1520-6106
1520-5207
DOI:10.1021/jp0375160