Kinetics and Mechanism of Substitution Reactions between Myoglobin and Activated Self-Assembled Monolayers Investigated Using Surface Plasmon Resonance

The rate of the reaction between solution-phase myoglobin and SAMs fabricated from ω-X-hexadecylthiomethylbenzenes where X was a mesylate, chloride, or bromide was investigated using surface plasmon resonance (spr). The data were fit to a model in which nonspecific adsorption competes with a second-...

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Bibliographic Details
Published in:Langmuir 2002-10, Vol.18 (21), p.8156-8160
Main Authors: Deckert, Alice A, Lesko, Jennifer, Todaro, Stephanie, Doyle, Margaret, Delaney, Christine
Format: Article
Language:English
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Summary:The rate of the reaction between solution-phase myoglobin and SAMs fabricated from ω-X-hexadecylthiomethylbenzenes where X was a mesylate, chloride, or bromide was investigated using surface plasmon resonance (spr). The data were fit to a model in which nonspecific adsorption competes with a second-order substitution reaction between myoglobin and the surface species. The second-order reaction rate constants obtained from the regression analysis were 440 ± 140 M-1s-1 for the bromide, 56 ± 10 M-1s-1 for the mesylate, and 38 ± 12 M-1s-1 for the chloride. The experimental rate law fit to the data and the increase in rate with decreased basicity of the leaving group were consistent with an SN2 mechanism.
ISSN:0743-7463
1520-5827
DOI:10.1021/la025767e