DNA Collisions with a Large, Conducting Post

We theoretically treat the problem of DNA collisions with large, ideally conducting posts during electrophoresis. We exploit a separation of time and length scales that allows the problem to be broken into two connected problems: (i) compression of a molecule against a flat wall by an applied transv...

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Bibliographic Details
Published in:Macromolecules 2010-06, Vol.43 (12), p.5424-5432
Main Authors: Trahan, Daniel W, Doyle, Patrick S
Format: Article
Language:English
Subjects:
Applied sciences
Exact sciences and technology
Miscellaneous
Natural polymers
Physicochemistry of polymers
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Summary:We theoretically treat the problem of DNA collisions with large, ideally conducting posts during electrophoresis. We exploit a separation of time and length scales that allows the problem to be broken into two connected problems: (i) compression of a molecule against a flat wall by an applied transverse field and (ii) transport of a molecule across the post surface due to both tangential diffusion and convection. We address the former using a combination of statistical mechanics and blob theory and the latter utilizing a Fokker−Planck approach. The theoretical predictions are then compared with the results of Brownian dynamics simulations.
ISSN:0024-9297
1520-5835
DOI:10.1021/ma100175t