Revisiting the Conformation and Dynamics of DNA in Slitlike Confinement

We experimentally investigated the equilibrium conformation and dynamics of single DNA molecules in slitlike nanochannels. We measured the in-plane radius of gyration (R ∥), diffusivity (D), and longest relaxation time (τ) of λ-DNA (48.5 kbp) as functions of the slit height using fluorescence micros...

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Bibliographic Details
Published in:Macromolecules 2010-09, Vol.43 (17), p.7368-7377
Main Authors: Tang, Jing, Levy, Stephen L, Trahan, Daniel W, Jones, Jeremy J, Craighead, Harold G, Doyle, Patrick S
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
In solution. Condensed state. Thin layers
Miscellaneous
Molecular biophysics
Natural polymers
Physico-chemical properties of biomolecules
Physicochemistry of polymers
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Summary:We experimentally investigated the equilibrium conformation and dynamics of single DNA molecules in slitlike nanochannels. We measured the in-plane radius of gyration (R ∥), diffusivity (D), and longest relaxation time (τ) of λ-DNA (48.5 kbp) as functions of the slit height using fluorescence microscopy. Our results show that the in-plane radius of gyration increases monotonically with decreasing slit height, in contrast to results from Bonthuis et al. but in agreement with our simulations and those of other groups. In strong confinement (slit height
ISSN:0024-9297
1520-5835
DOI:10.1021/ma101157x