Coil−Stretch Transition of DNA Molecules in Slitlike Confinement

We experimentally investigate the influence of slitlike confinement on the coil−stretch transition of single DNA molecules in a homogeneous planar elongational electric field. We observe a more gradual coil−stretch transition characterized by two distinct critical strain rates for DNA in confinement...

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Published in:Macromolecules 2010-03, Vol.43 (6), p.3081-3089
Main Authors: Tang, Jing, Trahan, Daniel W, Doyle, Patrick S
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
In solution. Condensed state. Thin layers
Miscellaneous
Molecular biophysics
Natural polymers
Physico-chemical properties of biomolecules
Physicochemistry of polymers
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creator Tang, Jing
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Doyle, Patrick S
description We experimentally investigate the influence of slitlike confinement on the coil−stretch transition of single DNA molecules in a homogeneous planar elongational electric field. We observe a more gradual coil−stretch transition characterized by two distinct critical strain rates for DNA in confinement, different from the unconfined case where a single critical strain rate exists. We postulate that the change in the coil−stretch transition is due to a modified spring law in confinement. We develop a dumbbell model to extract an effective spring law by following the relaxation of an initially stretched DNA. We then use this spring law and kinetic theory modeling to predict the extension and fluctuations of DNA in planar elongational fields. The model predicts that a two-stage coil−stretch transition emerges in confinement, in accord with experimental observations.
doi_str_mv 10.1021/ma902689c
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Applied sciences
Biological and medical sciences
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
In solution. Condensed state. Thin layers
Miscellaneous
Molecular biophysics
Natural polymers
Physico-chemical properties of biomolecules
Physicochemistry of polymers
title Coil−Stretch Transition of DNA Molecules in Slitlike Confinement
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