Atomic Force Microscopy Can Detect the Binding of Yeast Replication Factor C to DNA

Both DNA replication and DNA repair processes depend on the activity of numerous DNA polymerase accessory proteins. For example, the eukaryotic clamp loader replication factor C (RFC) is required to load the sliding DNA clamp, PCNA, onto DNA at primer/template junctions. Although the complete RFC-ca...

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Bibliographic Details
Published in:Nano letters 2003-01, Vol.3 (1), p.39-41
Main Authors: Green, Lisa, Schotanus, Mark, McAlear, Michael A, Howell, Elizabeth A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
Miscellaneous
Mycology
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Summary:Both DNA replication and DNA repair processes depend on the activity of numerous DNA polymerase accessory proteins. For example, the eukaryotic clamp loader replication factor C (RFC) is required to load the sliding DNA clamp, PCNA, onto DNA at primer/template junctions. Although the complete RFC-catalyzed loading reaction requires ATP hydrolysis, indirect assays such as surface plasmon resonance have indicated that RFC can bind to single-stranded DNA in the absence of ATP. We have used atomic force microscopy to verify directly that RFC from Saccharomyces cerevisiae can bind to single-stranded DNA in the absence of nucleotides.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl025831c