Novel Streptavidin-Functionalized Silicon Nanowire Arrays for CD4+ T Lymphocyte Separation

Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4+ T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity bi...

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Bibliographic Details
Published in:Nano letters 2010-08, Vol.10 (8), p.2877-2883
Main Authors: Kim, Sung Tae, Kim, Dong-Joo, Kim, Tae-Jin, Seo, Deok-Won, Kim, Tae-Hong, Lee, Seung-Yong, Kim, Kwanghee, Lee, Kyung-Mi, Lee, Sang-Kwon
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
CD4-Positive T-Lymphocytes - cytology
Cell Separation
Condensed matter: structure, mechanical and thermal properties
Cross-disciplinary physics: materials science
rheology
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties
Materials science
Methods. Procedures. Technologies
Mice
Nanocrystalline materials
Nanoscale materials and structures: fabrication and characterization
Nanowires
Others
Physics
Quantum wires
Silicon - chemistry
Streptavidin - chemistry
Surfaces and interfaces
thin films and whiskers (structure and nonelectronic properties)
Various methods and equipments
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Summary:Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4+ T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalized SiNW with biotin-labeled CD4+ T lymphocytes. Fabricated SiNW arrays easily separated the CD4+ T lymphocytes from the mouse whole splenocytes with over ∼88% purity and demonstrated tight attachment to CD4+ T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl100942p