Novel Streptavidin-Functionalized Silicon Nanowire Arrays for CD4+ T Lymphocyte Separation

• Published in: Nano letters 2010-08, Vol.10 (8), p.2877-2883
• Main Authors: Kim, Sung Tae, Kim, Dong-Joo, Kim, Tae-Jin, Seo, Deok-Won, Kim, Tae-Hong, Lee, Seung-Yong, Kim, Kwanghee, Lee, Kyung-Mi, Lee, Sang-Kwon
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Biotechnology; CD4-Positive T-Lymphocytes - cytology; Cell Separation; Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties; Materials science; Methods. Procedures. Technologies; Mice; Nanocrystalline materials; Nanoscale materials and structures: fabrication and characterization; Nanowires; Others; Physics; Quantum wires; Silicon - chemistry; Streptavidin - chemistry; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties); Various methods and equipments
• ISSN: 1530-6984; 1530-6992
• DOI: 10.1021/nl100942p

Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4+ T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalized SiNW with biotin-labeled CD4+ T lymphocytes. Fabricated SiNW arrays easily separated the CD4+ T lymphocytes from the mouse whole splenocytes with over ∼88% purity and demonstrated tight attachment to CD4+ T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.