A DNA-Damaging Oxoaporphine Alkaloid from Piper caninum

Bioassay-guided fractionation of an active organic extract of Piper caninum, using a sensitive yeast assay to monitor putative double-strand DNA-damaging activity, resulted in the isolation of the 4,5-dioxoaporphine alkaloid cepharadione A (1). Compound 1 exhibited potent inhibitory activity in a ye...

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2004-07, Vol.67 (7), p.1162-1164
Main Authors: Ma, Ji, Jones, Shannon H, Marshall, Rebekah, Johnson, Randall K, Hecht, Sidney M
Format: Article
Language:English
Subjects:
Alkaloids - chemistry
Alkaloids - isolation & purification
Alkaloids - pharmacology
Aporphines - chemistry
Aporphines - isolation & purification
Aporphines - pharmacology
Biological and medical sciences
DNA Damage
General pharmacology
Indonesia
Inhibitory Concentration 50
Medical sciences
Molecular Structure
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Piper - chemistry
Plant Stems - chemistry
Plants, Medicinal - chemistry
Saccharomyces cerevisiae - drug effects
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Summary:Bioassay-guided fractionation of an active organic extract of Piper caninum, using a sensitive yeast assay to monitor putative double-strand DNA-damaging activity, resulted in the isolation of the 4,5-dioxoaporphine alkaloid cepharadione A (1). Compound 1 exhibited potent inhibitory activity in a yeast cytotoxicity assay with IC50 values of 50.2 nM toward RS321NpRAD52 grown on glucose versus 293 nM toward the same yeast strain grown on galactose.
ISSN:0163-3864
1520-6025
DOI:10.1021/np040056x