Use of Potato Disc and Brine Shrimp Bioassays to Detect Activity and Isolate Piceatannol as the Antileukemic Principle from the Seeds of Euphorbia lagascae

EtOH extracts of the seeds of Euphorbia lagascae inhibited crown gall tumors on potato discs, were active against 3PS (in vivo) and 9PS (in vitro) mouse leukemia, and were toxic to brine shrimp. Using the potato disc and brine shrimp bioassays to monitor fractionation, piceatannol (3,4,3',5...

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 1984-03, Vol.47 (2), p.347-352
Main Authors: Ferrigni, N. R, McLaughlin, J. L, Powell, R. G, Smith, C. R
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents, Phytogenic - isolation & purification
Artemia - drug effects
Biological and medical sciences
Drug Evaluation, Preclinical - methods
General pharmacology
Leukemia - drug therapy
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Tumors
Plants, Medicinal - analysis
Stilbenes - isolation & purification
Stilbenes - pharmacology
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Summary:EtOH extracts of the seeds of Euphorbia lagascae inhibited crown gall tumors on potato discs, were active against 3PS (in vivo) and 9PS (in vitro) mouse leukemia, and were toxic to brine shrimp. Using the potato disc and brine shrimp bioassays to monitor fractionation, piceatannol (3,4,3',5'- tetrahydroxystilbene ) was isolated as the 3PS active principle. Aesculetin (6,7-dihydroxycoumarin) was isolated in large quantity, was weakly cytotoxic in 9KB , but was inactive in the other bioassay systems. Sucrose was also isolated. A combination of the simple crown gall and brine shrimp bioassays can thus be used both to detect and to isolate plant antitumor substances, minimizing the need for extensive antitumor testing in vivo.
ISSN:0163-3864
1520-6025
DOI:10.1021/np50032a019