New Fluoranthene FLUN-550 as a Fluorescent Probe for Selective Staining and Quantification of Intracellular Lipid Droplets

A new class of live cell permeant, nontoxic fluoranthene-based fluorescent probe (FLUN-550) having a high Stokes shift in aqueous medium has been discovered. It showed selective staining of lipid droplets (LDs, dynamic cytoplasmic organelles) at a low concentration without background noise in in vit...

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Bibliographic Details
Published in:Organic letters 2014-02, Vol.16 (3), p.756-759
Main Authors: Goel, Atul, Sharma, Ashutosh, Kathuria, Manoj, Bhattacharjee, Arindam, Verma, Ashwni, Mishra, Prabhat R, Nazir, Aamir, Mitra, Kalyan
Format: Article
Language:English
Subjects:
Animals
Antiprotozoal Agents - chemistry
Antiprotozoal Agents - pharmacology
Caenorhabditis elegans - chemistry
Caenorhabditis elegans - metabolism
Cell Line, Tumor
Fluorenes - chemistry
Fluorescent Dyes - chemistry
Humans
Lipids - analysis
Lipids - chemistry
Macrophages - chemistry
Macrophages - metabolism
Macrophages - parasitology
Molecular Structure
Polycyclic Compounds - chemistry
Staining and Labeling
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Summary:A new class of live cell permeant, nontoxic fluoranthene-based fluorescent probe (FLUN-550) having a high Stokes shift in aqueous medium has been discovered. It showed selective staining of lipid droplets (LDs, dynamic cytoplasmic organelles) at a low concentration without background noise in in vitro live cell imaging of 3T3-L1 preadipocytes, J774 macrophages, MCF7 breast cancer cells, and single-celled, parasitic protozoa Leishmania donovani promastigotes and in vivo nonparasitic soil nematode C. elegans.
ISSN:1523-7060
1523-7052
DOI:10.1021/ol403470d