Is It Essential to Use Anhydrous Acetonitrile in the Manufacture of Phosphorothioate Oligonucleotides?

It is demonstrated that acetonitrile containing up to at least 200 ppm water can be used as the wash solvent and to prepare the capping and sulfur transfer reagent solutions used in large-scale solid-phase phosphorothioate oligonucleotide synthesis, thus replacing most of the expensive anhydrous ace...

Full description

Saved in:
Bibliographic Details
Published in:Organic process research & development 1999-11, Vol.3 (6), p.485-487
Main Authors: Capaldi, Daniel C, Scozzari, Anthony N, Cole, Douglas L, Ravikumar, Vasulinga T
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:It is demonstrated that acetonitrile containing up to at least 200 ppm water can be used as the wash solvent and to prepare the capping and sulfur transfer reagent solutions used in large-scale solid-phase phosphorothioate oligonucleotide synthesis, thus replacing most of the expensive anhydrous acetonitrile previously used. No compromise in yield or product quality is observed, compared to a control synthesis performed using anhydrous acetonitrile throughout.
ISSN:1083-6160
1520-586X
DOI:10.1021/op9900333