Process Development for Purification of Therapeutic Antisense Oligonucleotides by Anion-Exchange Chromatography

This article describes the development of an anion-exchange chromatography method for purification of phosphorothioate antisense oligonucleotides. A variety of operating conditions were studied and optimized at small scale. These optimized parameters were used to generate data at multigram scale. Th...

Full description

Saved in:
Bibliographic Details
Published in:Organic process research & development 2000-05, Vol.4 (3), p.205-213
Main Authors: Deshmukh, Ranjit R, Miller, Jamie E, De Leon, Patricia, Leitch, William E, Cole, Douglas L, Sanghvi, Yogesh S
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This article describes the development of an anion-exchange chromatography method for purification of phosphorothioate antisense oligonucleotides. A variety of operating conditions were studied and optimized at small scale. These optimized parameters were used to generate data at multigram scale. The selected method can give a high purity product at a very good yield of the full-length desired product. This method can be suitably scaled up for antisense oligonucleotide purification at large scales.
ISSN:1083-6160
1520-586X
DOI:10.1021/op990091o