Serum and Urinary Metabonomic Study of Human Osteosarcoma

Osteosarcoma (OS) is the most common malignant bone tumor found in children. Currently, researchers have focused on protein and gene levels, while the associated metabolic variations have been poorly understood. In this study, we used a gas chromatography mass spectrometry approach and profiled smal...

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Bibliographic Details
Published in:Journal of proteome research 2010-09, Vol.9 (9), p.4861-4868
Main Authors: Zhang, Zhiyu, Qiu, Yunping, Hua, Yingqi, Wang, Yihuang, Chen, Tianlu, Zhao, Aihua, Chi, Yi, Pan, Li, Hu, Shuo, Li, Jian, Yang, Chengwei, Li, Guodong, Sun, Wei, Cai, Zhengdong, Jia, Wei
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Amino Acids - blood
Amino Acids - urine
Biomarkers, Tumor - blood
Biomarkers, Tumor - urine
Bone Neoplasms - blood
Bone Neoplasms - urine
Case-Control Studies
Discriminant Analysis
Female
Gas Chromatography-Mass Spectrometry
Hippurates - blood
Hippurates - urine
Humans
Least-Squares Analysis
Male
Metabolic Networks and Pathways
Metabolomics - methods
Middle Aged
Osteosarcoma - blood
Osteosarcoma - urine
Principal Component Analysis
Putrescine - blood
Putrescine - urine
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Summary:Osteosarcoma (OS) is the most common malignant bone tumor found in children. Currently, researchers have focused on protein and gene levels, while the associated metabolic variations have been poorly understood. In this study, we used a gas chromatography mass spectrometry approach and profiled small-molecule metabolites in serum and urine of 24 OS patients, 19 benign bone tumor patients, and 32 healthy controls, to determine whether there are significant alterations associated with carcinogenesis. The metabonomic results demonstrate clear intergroup separations between healthy control subjects and bone tumor patients in the orthogonal partial least-squares-discriminant analysis (OPLS-DA) models. Differential metabolites identified from the metabonomic analysis suggest a disrupted energy metabolism in OS patients, as characterized by significantly down-regulated TCA cycle and glycolysis, down-regulated lipid metabolism, dysregulated sugar levels, and up-regulated amino acid metabolism. Additionally, an increased activity of glutathione metabolism, and increased polyamine metabolism also contributed to a characteristic metabolic signature of OS patients.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr100480r