Human Glutathione Transferases Catalyzing the Conjugation of the Hepatoxin Microcystin-LR

• Published in: Chemical research in toxicology 2011-06, Vol.24 (6), p.926-933
• Main Authors: Buratti, Franca M, Scardala, Simona, Funari, Enzo, Testai, Emanuela
• Format: Article
• Language: English
• Subjects: Bacterial Toxins - isolation & purification; Bacterial Toxins - metabolism; Bacterial Toxins - pharmacokinetics; Escherichia coli - genetics; Gene Expression; Glutathione - metabolism; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Humans; Inactivation, Metabolic; Microcystins - isolation & purification; Microcystins - metabolism; Microcystins - pharmacokinetics; Microcystis - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism
• ISSN: 0893-228X; 1520-5010
• DOI: 10.1021/tx2000976

Many cyanobacterial species are able to produce cyanotoxins as secondary metabolites. Among them, microcystins (MC) are a group of around 80 congeners of toxic cyclic heptapeptides. MC-LR is the most studied MC congener, in view of its high acute hepatotoxicity and tumor promoting activity. Humans may be exposed to cyanotoxins through several routes, the oral one being the most important. The accepted pathway for MC-LR detoxication and excretion in the urine is GSH conjugation. The GSH adduct (GS-MCLR) formation has been shown to occur spontaneously and enzymatically, catalyzed by glutathione transferases (GSTs). The enzymatic reaction has been reported but not characterized both in vitro and in vivo in animal and plant species. No data are available on humans. In the present work, the MC-LR conjugation with GSH catalyzed by five recombinant human GSTs (A1-1, A3-3, M1-1, P1-1, and T1-1) has been characterized for the first time. All GSTs are able to catalyze the reaction; kinetic parameters K m, k cat, and their relative specific activities to form GS-MCLR were derived (T1-1 > A1-1 > M1-1 > A3-3 ≫ P1-1). In the range of MC tested concentrations used (0.25–50 μM) GSTT1-1 and A1-1 showed a typical saturation curve with similar affinity for MC-LR (≈80 μM; k cat values 0.18 and 0.10 min–1, respectively), A3-3 and M1-1 were linear, whereas GSTP1-1 showed a temperature-dependent sigmoidal allosteric curve with a k cat = 0.11 min–1. The enzymes mainly expressed in the liver and gastrointestinal tract, GSTA1-1, T1-1, and M1-1, seemed to be mainly involved in the MC-LR detoxification after oral exposure, whereas P1-1 kinetics and location in the skin suggest a role related to dermal exposure. Considering the high frequency of some GST polymorphism, especially M1 and T1 gene deletion, with complete loss in activity, this information could be the first step to identify groups of individual at higher risk associated with MC exposure.