A New Method for the Detection of Peptides and Similar Compounds on Paper Chromatograms

SINCE the first introduction 1 of paper chromatography for the separation and identification of amino-acids and peptides, ninhydrin has been used almost exclusively as the spraying reagent to reveal the positions of the spots, although some use has been made of ultra-violet fluorescence 2 and of spr...

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Bibliographic Details
Published in:Nature (London) 1952-05, Vol.169 (4309), p.922-923
Main Authors: RYDON, H. N., SMITH, P. W. G.
Format: Article
Language:English
Subjects:
Humanities and Social Sciences
letter
multidisciplinary
Science
Science (multidisciplinary)
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Summary:SINCE the first introduction 1 of paper chromatography for the separation and identification of amino-acids and peptides, ninhydrin has been used almost exclusively as the spraying reagent to reveal the positions of the spots, although some use has been made of ultra-violet fluorescence 2 and of spraying with potassium permanganate 3 and specific reagents for individual amino-acids 4 . Ninhydrin, however, has two serious limitations: it does not react with cyclic peptides or with acylated amino-acids or peptides, while with peptides, since only the terminal amino-group enters into the reaction, the colour developed with a given amount of material decreases with increasing molecular weight.
ISSN:0028-0836
1476-4687
DOI:10.1038/169922b0