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Methyl Proline in Young Apple Fruits
IT was recently suggested that a ‘new’ amino-acid isolated from the apple fruit was a methyl proline 1 . This acid (called D 1 in the original communication) has now been compared with an authentic specimen of γ-methyl proline kindly supplied by Prof. F. C. Steward, of Cornell University. The positi...
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| Published in: | Nature (London) 1954-03, Vol.173 (4404), p.588-589 |
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| creator | HULME, A. C ARTHINGTON, W |
| description | IT was recently suggested that a ‘new’ amino-acid isolated from the apple fruit was a methyl proline
1
. This acid (called
D
1
in the original communication) has now been compared with an authentic specimen of γ-methyl proline kindly supplied by Prof. F. C. Steward, of Cornell University. The positions occupied by these two acids on two-dimensional chromatograms (phenol ammonia–butanol–acetic acid–water) were found to be identical. Mr. J. K. Pollard in Prof. Steward's laboratory has also found the two compounds to superimpose on collidine–lutidine: phenol chromatograms. The two compounds (
D
1
and γ-methyl proline) give identical colour reactions on paper chromatograms with ninhydrin (a yellow-orange colour) and with isatin (a pure blue colour developing at the same rate with both acids). Not more than ∼ 17 mgm. of
D
1
were obtained from 70 kgm. of young Worcester Pearmain apples, and elementary analysis on a sample containing traces of inorganic salt gave the following: C, 53.6 per cent; H, 8.17 per cent ; N, 9.7 per cent. Assuming this indicates a C
6
compound with an empirical formula of C
6
H
11
O
2
N, and correcting the analyses for ash gives: C, 55.8; H, 8.5; N, 10.1; methyl proline requires : C, 55.8; H, 8.5; N, 10.8 per cent. |
| doi_str_mv | 10.1038/173588b0 |
| format | article |
| fullrecord | <record><control><sourceid>nature_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1038_173588b0</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>173588b0</sourcerecordid><originalsourceid>FETCH-LOGICAL-c282t-24ce9353e5dbfe1e4bed65f47cb92d511ad304e3ce979c884cb1da40f9ab81f83</originalsourceid><addsrcrecordid>eNptzzFPwzAUBGALgUQoSPwClIEBhsBzbMfOWFUtIBXBAANTZDvPJVVwKjsZ-u8JChUL0y2fTneEXFK4o8DUPZVMKGXgiCSUyyLjhZLHJAHIVQaKFafkLMYtAAgqeUKun7H_3Lfpa-jaxmPa-PSjG_wmne92LaarMDR9PCcnTrcRL35zRt5Xy7fFY7Z-eXhazNeZzVXeZzm3WDLBUNTGIUVusC6E49KaMq8FpbpmwJGNSpZWKW4NrTUHV2qjqFNsRm6mXhu6GAO6aheaLx32FYXq5111eDfS24nGkfgNhmrbDcGP6_6zV5P1uh8C_pUewDesfVhL</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Methyl Proline in Young Apple Fruits</title><source>North East Research Libraries Nature Academic Titles</source><creator>HULME, A. C ; ARTHINGTON, W</creator><creatorcontrib>HULME, A. C ; ARTHINGTON, W</creatorcontrib><description>IT was recently suggested that a ‘new’ amino-acid isolated from the apple fruit was a methyl proline
1
. This acid (called
D
1
in the original communication) has now been compared with an authentic specimen of γ-methyl proline kindly supplied by Prof. F. C. Steward, of Cornell University. The positions occupied by these two acids on two-dimensional chromatograms (phenol ammonia–butanol–acetic acid–water) were found to be identical. Mr. J. K. Pollard in Prof. Steward's laboratory has also found the two compounds to superimpose on collidine–lutidine: phenol chromatograms. The two compounds (
D
1
and γ-methyl proline) give identical colour reactions on paper chromatograms with ninhydrin (a yellow-orange colour) and with isatin (a pure blue colour developing at the same rate with both acids). Not more than ∼ 17 mgm. of
D
1
were obtained from 70 kgm. of young Worcester Pearmain apples, and elementary analysis on a sample containing traces of inorganic salt gave the following: C, 53.6 per cent; H, 8.17 per cent ; N, 9.7 per cent. Assuming this indicates a C
6
compound with an empirical formula of C
6
H
11
O
2
N, and correcting the analyses for ash gives: C, 55.8; H, 8.5; N, 10.1; methyl proline requires : C, 55.8; H, 8.5; N, 10.8 per cent.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/173588b0</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Humanities and Social Sciences ; letter ; multidisciplinary ; Science ; Science (multidisciplinary)</subject><ispartof>Nature (London), 1954-03, Vol.173 (4404), p.588-589</ispartof><rights>Springer Nature Limited 1954</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-24ce9353e5dbfe1e4bed65f47cb92d511ad304e3ce979c884cb1da40f9ab81f83</citedby><cites>FETCH-LOGICAL-c282t-24ce9353e5dbfe1e4bed65f47cb92d511ad304e3ce979c884cb1da40f9ab81f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids></links><search><creatorcontrib>HULME, A. C</creatorcontrib><creatorcontrib>ARTHINGTON, W</creatorcontrib><title>Methyl Proline in Young Apple Fruits</title><title>Nature (London)</title><addtitle>Nature</addtitle><description>IT was recently suggested that a ‘new’ amino-acid isolated from the apple fruit was a methyl proline
1
. This acid (called
D
1
in the original communication) has now been compared with an authentic specimen of γ-methyl proline kindly supplied by Prof. F. C. Steward, of Cornell University. The positions occupied by these two acids on two-dimensional chromatograms (phenol ammonia–butanol–acetic acid–water) were found to be identical. Mr. J. K. Pollard in Prof. Steward's laboratory has also found the two compounds to superimpose on collidine–lutidine: phenol chromatograms. The two compounds (
D
1
and γ-methyl proline) give identical colour reactions on paper chromatograms with ninhydrin (a yellow-orange colour) and with isatin (a pure blue colour developing at the same rate with both acids). Not more than ∼ 17 mgm. of
D
1
were obtained from 70 kgm. of young Worcester Pearmain apples, and elementary analysis on a sample containing traces of inorganic salt gave the following: C, 53.6 per cent; H, 8.17 per cent ; N, 9.7 per cent. Assuming this indicates a C
6
compound with an empirical formula of C
6
H
11
O
2
N, and correcting the analyses for ash gives: C, 55.8; H, 8.5; N, 10.1; methyl proline requires : C, 55.8; H, 8.5; N, 10.8 per cent.</description><subject>Humanities and Social Sciences</subject><subject>letter</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1954</creationdate><recordtype>article</recordtype><recordid>eNptzzFPwzAUBGALgUQoSPwClIEBhsBzbMfOWFUtIBXBAANTZDvPJVVwKjsZ-u8JChUL0y2fTneEXFK4o8DUPZVMKGXgiCSUyyLjhZLHJAHIVQaKFafkLMYtAAgqeUKun7H_3Lfpa-jaxmPa-PSjG_wmne92LaarMDR9PCcnTrcRL35zRt5Xy7fFY7Z-eXhazNeZzVXeZzm3WDLBUNTGIUVusC6E49KaMq8FpbpmwJGNSpZWKW4NrTUHV2qjqFNsRm6mXhu6GAO6aheaLx32FYXq5111eDfS24nGkfgNhmrbDcGP6_6zV5P1uh8C_pUewDesfVhL</recordid><startdate>19540327</startdate><enddate>19540327</enddate><creator>HULME, A. C</creator><creator>ARTHINGTON, W</creator><general>Nature Publishing Group UK</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19540327</creationdate><title>Methyl Proline in Young Apple Fruits</title><author>HULME, A. C ; ARTHINGTON, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-24ce9353e5dbfe1e4bed65f47cb92d511ad304e3ce979c884cb1da40f9ab81f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1954</creationdate><topic>Humanities and Social Sciences</topic><topic>letter</topic><topic>multidisciplinary</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HULME, A. C</creatorcontrib><creatorcontrib>ARTHINGTON, W</creatorcontrib><collection>CrossRef</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HULME, A. C</au><au>ARTHINGTON, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methyl Proline in Young Apple Fruits</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><date>1954-03-27</date><risdate>1954</risdate><volume>173</volume><issue>4404</issue><spage>588</spage><epage>589</epage><pages>588-589</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>IT was recently suggested that a ‘new’ amino-acid isolated from the apple fruit was a methyl proline
1
. This acid (called
D
1
in the original communication) has now been compared with an authentic specimen of γ-methyl proline kindly supplied by Prof. F. C. Steward, of Cornell University. The positions occupied by these two acids on two-dimensional chromatograms (phenol ammonia–butanol–acetic acid–water) were found to be identical. Mr. J. K. Pollard in Prof. Steward's laboratory has also found the two compounds to superimpose on collidine–lutidine: phenol chromatograms. The two compounds (
D
1
and γ-methyl proline) give identical colour reactions on paper chromatograms with ninhydrin (a yellow-orange colour) and with isatin (a pure blue colour developing at the same rate with both acids). Not more than ∼ 17 mgm. of
D
1
were obtained from 70 kgm. of young Worcester Pearmain apples, and elementary analysis on a sample containing traces of inorganic salt gave the following: C, 53.6 per cent; H, 8.17 per cent ; N, 9.7 per cent. Assuming this indicates a C
6
compound with an empirical formula of C
6
H
11
O
2
N, and correcting the analyses for ash gives: C, 55.8; H, 8.5; N, 10.1; methyl proline requires : C, 55.8; H, 8.5; N, 10.8 per cent.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><doi>10.1038/173588b0</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1954-03, Vol.173 (4404), p.588-589 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_crossref_primary_10_1038_173588b0 |
| source | North East Research Libraries Nature Academic Titles |
| subjects | Humanities and Social Sciences letter multidisciplinary Science Science (multidisciplinary) |
| title | Methyl Proline in Young Apple Fruits |
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