Occurrence of an L(+)-Alanine-Dehydrogenase in Bacillus subtilis

THE metabolic analysis of mutant strains of B. subtilis has shown that the ability to use the ammonium ion as the sole source of nitrogen for growth corresponds to the presence of a glutamic-dehydrogenase 1 . However, a mutant unable to use the ammonium ion (strain S − ), lacking glutamic-dehydrogen...

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Bibliographic Details
Published in:Nature (London) 1955-12, Vol.176 (4492), p.1073-1075
Main Authors: WIAME, J. M, PIÉRARD, A
Format: Article
Language:English
Subjects:
Humanities and Social Sciences
letter
multidisciplinary
Science
Science (multidisciplinary)
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Summary:THE metabolic analysis of mutant strains of B. subtilis has shown that the ability to use the ammonium ion as the sole source of nitrogen for growth corresponds to the presence of a glutamic-dehydrogenase 1 . However, a mutant unable to use the ammonium ion (strain S − ), lacking glutamic-dehydrogenase, oxidizes glutamic acid very actively. This strain may grow, with aeration, in a culture medium containing: l (+)-glutamate 0.01 M , ammonium sulphate 0.01 M , phosphate buffer (KH 2 PO 4 –Na 2 HPO 4 ) 0.02 M , p H 7.5, sodium chloride 0.01 M , potassium sulphate 0.01 M , magnesium sulphate 0.001 M and ferric citrate 0.0004 M .
ISSN:0028-0836
1476-4687
DOI:10.1038/1761073b0